A Persulfidation-Based Protein Modification Controls Guard Cell ABA Signaling
80 Pages Posted: 23 Jul 2019 Sneak Peek Status: Review CompleteMore...
Hydrogen sulfide (H2S) is a gaseous signaling molecule that regulates diverse cellular signaling through post translational modifications (PTM) of specific cysteine residues to form persulfides. However, this important redox-based PTM remains poorly understood. We have therefore analyzed how protein persulfidation is utilized as a specific, reversible signaling mechanism. Here we show the reciprocal control between DES1-produced H2S and guard cell autonomous ABA synthesis in regulation of stomatal water loss. ABA stimulates DES1 itself persulfidation at Cys44/205 in a redox-dependent manner. Moreover, sustained H2S accumulation drives persulfidation of the RBOHD protein at Cys825/890, enhancing ROS-production. Together, these processes form a negative feedback loop that fine-tunes guard cell redox homeostasis and ABA signaling. These findings not only expand our current knowledge of H2S function in the context of ABA metabolism and signaling, but also demonstrate the presence of a rapid signal integration mechanism with regard to specific and reversible redox-based PTMs in response to changing environmental conditions.
Keywords: hydrogen sulfide, persulfidation, DES1, RBOHD, ABA signal, guard cell
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