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A Persulfidation-Based Protein Modification Controls Guard Cell ABA Signaling

80 Pages Posted: 23 Jul 2019 Sneak Peek Status: Review Complete

See all articles by Jie Shen

Jie Shen

Nanjing Agricultural University (NAU) - Laboratory Center of Life Sciences

Jing Zhang

Nanjing Agricultural University (NAU) - Laboratory Center of Life Sciences

Can Zhou

Nanjing Agricultural University (NAU) - Laboratory Center of Life Sciences

Mingjian Zhou

Nanjing Agricultural University (NAU) - Laboratory Center of Life Sciences

Heng Zhou

Nanjing Agricultural University (NAU) - Laboratory Center of Life Sciences

Beimi Cui

University of Edinburgh - Institute of Molecular Plant Sciences

Cecilia Gotor

University of Seville

Luis C. Romero

University of Seville

Christine Helen Foyer

University of Leeds - Centre for Plant Sciences

Qiaona Pan

University of Edinburgh - Institute of Molecular Plant Sciences

Deliang Wu

Jiangsu Academy of Agricultural Sciences

Xianchao Yin

Jiangsu Academy of Agricultural Sciences

Xin Liu

Qingdao Agricultural University

Wenbiao Shen

Nanjing Agricultural University (NAU) - Laboratory Center of Life Sciences

Weiti Cui

Nanjing Agricultural University (NAU) - Laboratory Center of Life Sciences

Yanjie Xie

Nanjing Agricultural University (NAU) - Laboratory Center of Life Sciences

More...

Abstract

Hydrogen sulfide (H2S) is a gaseous signaling molecule that regulates diverse cellular signaling through post translational modifications (PTM) of specific cysteine residues to form persulfides. However, this important redox-based PTM remains poorly understood. We have therefore analyzed how protein persulfidation is utilized as a specific, reversible signaling mechanism. Here we show the reciprocal control between DES1-produced H2S and guard cell autonomous ABA synthesis in regulation of stomatal water loss. ABA stimulates DES1 itself persulfidation at Cys44/205 in a redox-dependent manner. Moreover, sustained H2S accumulation drives persulfidation of the RBOHD protein at Cys825/890, enhancing ROS-production. Together, these processes form a negative feedback loop that fine-tunes guard cell redox homeostasis and ABA signaling. These findings not only expand our current knowledge of H2S function in the context of ABA metabolism and signaling, but also demonstrate the presence of a rapid signal integration mechanism with regard to specific and reversible redox-based PTMs in response to changing environmental conditions.

Keywords: hydrogen sulfide, persulfidation, DES1, RBOHD, ABA signal, guard cell

Suggested Citation

Shen, Jie and Zhang, Jing and Zhou, Can and Zhou, Mingjian and Zhou, Heng and Cui, Beimi and Gotor, Cecilia and Romero, Luis C. and Foyer, Christine Helen and Pan, Qiaona and Wu, Deliang and Yin, Xianchao and Liu, Xin and Shen, Wenbiao and Cui, Weiti and Xie, Yanjie, A Persulfidation-Based Protein Modification Controls Guard Cell ABA Signaling (July 22, 2019). CELL-REPORTS-D-19-02762. Available at SSRN: https://ssrn.com/abstract=3424315 or http://dx.doi.org/10.2139/ssrn.3424315
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Jie Shen

Nanjing Agricultural University (NAU) - Laboratory Center of Life Sciences

China

Jing Zhang

Nanjing Agricultural University (NAU) - Laboratory Center of Life Sciences

China

Can Zhou

Nanjing Agricultural University (NAU) - Laboratory Center of Life Sciences ( email )

China

Mingjian Zhou

Nanjing Agricultural University (NAU) - Laboratory Center of Life Sciences

China

Heng Zhou

Nanjing Agricultural University (NAU) - Laboratory Center of Life Sciences

China

Beimi Cui

University of Edinburgh - Institute of Molecular Plant Sciences

Edinburgh, Scotland
United Kingdom

Cecilia Gotor

University of Seville

Avda. del Cid s/n
Sevilla, Sevilla 41004
Spain

Luis C. Romero

University of Seville

Avda. del Cid s/n
Sevilla, Sevilla 41004
Spain

Christine Helen Foyer

University of Leeds - Centre for Plant Sciences

Leeds, LS2 9JT
United Kingdom

Qiaona Pan

University of Edinburgh - Institute of Molecular Plant Sciences

Edinburgh, Scotland
United Kingdom

Deliang Wu

Jiangsu Academy of Agricultural Sciences

50 Zhongling Street
Nanjing, Jiangsu 210014
China

Xianchao Yin

Jiangsu Academy of Agricultural Sciences

50 Zhongling Street
Nanjing, Jiangsu 210014
China

Xin Liu

Qingdao Agricultural University

China

Wenbiao Shen

Nanjing Agricultural University (NAU) - Laboratory Center of Life Sciences

China

Weiti Cui

Nanjing Agricultural University (NAU) - Laboratory Center of Life Sciences

China

Yanjie Xie (Contact Author)

Nanjing Agricultural University (NAU) - Laboratory Center of Life Sciences ( email )

China

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