puc-header

Complete Whole-Brain Single Neuron Reconstruction Reveals Morphological Diversity in Molecularly Defined Claustral and Cortical Neuron Types

51 Pages Posted: 30 Jul 2019 Sneak Peek Status: Review Complete

See all articles by Yun Wang

Yun Wang

Allen Institute for Brain Science

Peng Xie

Allen Institute for Brain Science

Hui Gong

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Britton Chance Center for Biomedical Photonics

Zhi Zhou

Allen Institute for Brain Science

Xiuli Kuang

Wenzhou Medical University - School of Optometry and Ophthalmology

Yimin Wang

Shanghai University - School of Computer Engineering and Science

Anan Li

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Britton Chance Center for Biomedical Photonics

Yaoyao Li

Wenzhou Medical University - School of Optometry and Ophthalmology

Lijuan Liu

Southeast University (China) - Institute for Brain and Intelligence

Matthew B. Veldman

University of California, Los Angeles (UCLA) - Center for Neurobehavioral Genetics

Tanya L. Daigle

Allen Institute for Brain Science

Karla E. Hirokawa

Allen Institute for Brain Science

Lei Qu

Anhui University - Key Laboratory of Intelligent Computation & Signal Processing

Philip Lesnar

Allen Institute for Brain Science

Shengdian Jiang

Southeast University (China) - Institute for Brain and Intelligence

Yang Yu

Allen Institute for Brain Science

Wayne Wakeman

Allen Institute for Brain Science

Shaoqun Zeng

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Britton Chance Center for Biomedical Photonics

Xiangning Li

Chinese Academy of Sciences (CAS) - Center for Excellence in Brain Science and Intelligence Technology; Huazhong University of Science and Technology, School of Engineering Sciences, Wuhan National Laboratory for Optoelectronics, Britton Chance Center for Biomedical Photonics

Jing Yuan

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Britton Chance Center for Biomedical Photonics

Thuc Nghi Nguyen

Allen Institute for Brain Science

Rachael Larsen

Allen Institute for Brain Science

Sara Kebede

Allen Institute for Brain Science

Yuanyuan Song

Southeast University (China) - Institute for Brain and Intelligence

Lulu Ying

Southeast University (China) - Institute for Brain and Intelligence

Sujun Zhao

Southeast University (China) - Institute for Brain and Intelligence

Aaron Feiner

Allen Institute for Brain Science

Elise Shen

Allen Institute for Brain Science

Chris Hill

Allen Institute for Brain Science

Quanxin Wang

Allen Institute for Brain Science

Stephanie Mok

Allen Institute for Brain Science

Susan M. Sunkin

Allen Institute for Brain Science

Z. Josh Huang

Cold Spring Harbor Laboratory

Luke Esposito

Allen Institute for Brain Science

Zizhen Yao

Allen Institute for Brain Science

Michael J. Hawrylycz

Allen Institute for Brain Science

Bosiljka Tasic

Allen Institute for Brain Science

Lydia Ng

Allen Institute for Brain Science

Staci A. Sorensen

Allen Institute for Brain Science

X. William Yang

University of California, Los Angeles (UCLA) - Center for Neurobehavioral Genetics

Julie A. Harris

Allen Institute for Brain Science

Christof Koch

Allen Institute for Brain Science

Qingming Luo

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Britton Chance Center for Biomedical Photonics

Hanchuan Peng

Allen Institute for Brain Science

Hongkui Zeng

Allen Institute for Brain Science

More...

Abstract

Dendritic and axonal morphology is a defining feature of neuronal types and their connectivity. Yet our knowledge concerning the diversity of neuronal morphology is extremely limited. To systematically obtain single neuron full morphology on a brain-wide scale in mice, we established a pipeline that encompasses five major components: sparse labeling, whole-brain imaging, reconstruction, registration, and classification. We achieved sparse, robust and consistent fluorescent labeling by combining transgenic or viral Cre delivery with novel transgenic reporter lines, and generated whole-brain fluorescent imaging datasets containing tens of thousands of reconstructable neurons. We developed software tools for large-volume image data processing and computer-assisted morphological reconstruction. For a proof-of-principle, we reconstructed the full morphologies of 96 neurons from the claustrum and cortex that belong to a single transcriptomically-defined subclass, and classified them into multiple morphological types, suggesting that they work in a targeted and coordinated manner to process cortical information over a large region.

Suggested Citation

Wang, Yun and Xie, Peng and Gong, Hui and Zhou, Zhi and Kuang, Xiuli and Wang, Yimin and Li, Anan and Li, Yaoyao and Liu, Lijuan and Veldman, Matthew B. and Daigle, Tanya L. and Hirokawa, Karla E. and Qu, Lei and Lesnar, Philip and Jiang, Shengdian and Yu, Yang and Wakeman, Wayne and Zeng, Shaoqun and Li, Xiangning and Yuan, Jing and Nguyen, Thuc Nghi and Larsen, Rachael and Kebede, Sara and Song, Yuanyuan and Ying, Lulu and Zhao, Sujun and Feiner, Aaron and Shen, Elise and Hill, Chris and Wang, Quanxin and Mok, Stephanie and Sunkin, Susan M. and Huang, Z. Josh and Esposito, Luke and Yao, Zizhen and Hawrylycz, Michael J. and Tasic, Bosiljka and Ng, Lydia and Sorensen, Staci A. and Yang, X. William and Harris, Julie A. and Koch, Christof and Luo, Qingming and Peng, Hanchuan and Zeng, Hongkui, Complete Whole-Brain Single Neuron Reconstruction Reveals Morphological Diversity in Molecularly Defined Claustral and Cortical Neuron Types (July 26, 2019). Available at SSRN: https://ssrn.com/abstract=3427280 or http://dx.doi.org/10.2139/ssrn.3427280
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Yun Wang

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Peng Xie

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Hui Gong

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Britton Chance Center for Biomedical Photonics

Wuhan
China

Zhi Zhou

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Xiuli Kuang

Wenzhou Medical University - School of Optometry and Ophthalmology

Wenzhou
China

Yimin Wang

Shanghai University - School of Computer Engineering and Science

149 Yanchang Road
Shangda Road 99
Shanghai 200072, Shanghai 200444
China

Anan Li

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Britton Chance Center for Biomedical Photonics

Wuhan
China

Yaoyao Li

Wenzhou Medical University - School of Optometry and Ophthalmology

Wenzhou
China

Lijuan Liu

Southeast University (China) - Institute for Brain and Intelligence

Nanjing
China

Matthew B. Veldman

University of California, Los Angeles (UCLA) - Center for Neurobehavioral Genetics

Los Angeles, CA
United States

Tanya L. Daigle

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Karla E. Hirokawa

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Lei Qu

Anhui University - Key Laboratory of Intelligent Computation & Signal Processing

Anhui
China

Philip Lesnar

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Shengdian Jiang

Southeast University (China) - Institute for Brain and Intelligence

Nanjing
China

Yang Yu

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Wayne Wakeman

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Shaoqun Zeng

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Britton Chance Center for Biomedical Photonics

Wuhan
China

Xiangning Li

Chinese Academy of Sciences (CAS) - Center for Excellence in Brain Science and Intelligence Technology

Shanghai, 200031
China

Huazhong University of Science and Technology, School of Engineering Sciences, Wuhan National Laboratory for Optoelectronics, Britton Chance Center for Biomedical Photonics

Wuhan
China

Jing Yuan

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Britton Chance Center for Biomedical Photonics

Wuhan
China

Thuc Nghi Nguyen

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Rachael Larsen

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Sara Kebede

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Yuanyuan Song

Southeast University (China) - Institute for Brain and Intelligence

Nanjing
China

Lulu Ying

Southeast University (China) - Institute for Brain and Intelligence

Nanjing
China

Sujun Zhao

Southeast University (China) - Institute for Brain and Intelligence

Nanjing
China

Aaron Feiner

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Elise Shen

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Chris Hill

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Quanxin Wang

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Stephanie Mok

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Susan M. Sunkin

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Z. Josh Huang

Cold Spring Harbor Laboratory

Cold Spring Harbor, NY 11724
United States

Luke Esposito

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Zizhen Yao

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Michael J. Hawrylycz

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Bosiljka Tasic

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Lydia Ng

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Staci A. Sorensen

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

X. William Yang

University of California, Los Angeles (UCLA) - Center for Neurobehavioral Genetics

Los Angeles, CA
United States

Julie A. Harris

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Christof Koch

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Qingming Luo

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Britton Chance Center for Biomedical Photonics

Wuhan
China

Hanchuan Peng

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Hongkui Zeng (Contact Author)

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Click here to go to Cell.com

Paper statistics

Abstract Views
514
Downloads
33