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DNA Catalyzed Efficient Production of Single-Stranded DNA Nanostructures

58 Pages Posted: 31 Jul 2019 Sneak Peek Status: Review Complete

See all articles by Youli Jia

Youli Jia

Fudan University - Institutes of Biomedical Sciences

Jin Liu

Fudan University - Institutes of Biomedical Sciences

Liman Chen

Fudan University - Institutes of Biomedical Sciences

Wei Li

Fudan University - Institutes of Biomedical Sciences

Hongzhou Gu

Fudan University - Institutes of Biomedical Sciences; Fudan University - Shanghai Institute of Cardiovascular Diseases; Fudan University - Center for Biotechnology and Biomedical Engineering

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Abstract

Single-stranded (ss) DNA nanostructures, created by unimolecular folding of kilo-based ssDNA, represent a novel class of DNA constructs suitable for biomaterial and biomedical applications. Here, using DNA-cleaving DNA enzymes (deoxyribozymes), we present a strategy for efficient and cost-effective production of milligrams of ssDNA nanostructures. We designed “genes” of ssDNA nanostructures flanked by self-cleaving deoxyribozymes and amplified the genes in the single-stranded form. Upon autoprocessing, the resulting amplicons release quadrillion copies of the ssDNA that self-folds into the desired structures. By engineering trans-acting deoxyribozymes, we showed that several ssDNA nanostructures of different shapes can be encoded and amplified in tandem with controllable release of specific shapes from the amplicons, thereby providing a convenient yet economical way to safely store “genetic codes” of nanostructures on an ssDNA for selective mass production on demand. We expect our approach will promote the development of applications of DNA catalysts as well as gene-sized ssDNA in broad areas.

Keywords: kilo-based single-stranded DNA, DNA catalyst, mass production, ssDNA nanostructure

Suggested Citation

Jia, Youli and Liu, Jin and Chen, Liman and Li, Wei and Gu, Hongzhou, DNA Catalyzed Efficient Production of Single-Stranded DNA Nanostructures (July 30, 2019). Available at SSRN: https://ssrn.com/abstract=3429156 or http://dx.doi.org/10.2139/ssrn.3429156
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Youli Jia

Fudan University - Institutes of Biomedical Sciences ( email )

138 Yi Xue Yuan Rd
Shanghai
China

Jin Liu

Fudan University - Institutes of Biomedical Sciences

Shanghai, 200032
China

Liman Chen

Fudan University - Institutes of Biomedical Sciences ( email )

138 Yi Xue Yuan Rd
Shanghai
China

Wei Li

Fudan University - Institutes of Biomedical Sciences

Shanghai, 200032
China

Hongzhou Gu (Contact Author)

Fudan University - Institutes of Biomedical Sciences ( email )

Shanghai, 200032
China

Fudan University - Shanghai Institute of Cardiovascular Diseases ( email )

China

Fudan University - Center for Biotechnology and Biomedical Engineering ( email )

Shanghai, 200032
China

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