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Generation of foxn1/ Casper Mutant Zebrafish for Allograft and Xenograft of Normal and Malignant Cells

42 Pages Posted: 21 Aug 2019 Sneak Peek Status: Review Complete

See all articles by Shuai Gao

Shuai Gao

Chinese Academy of Sciences (CAS) - State Key Laboratory of Membrane Biology

Peng Lv

Chinese Academy of Sciences (CAS), Kunming Institute of Zoology, State Key Laboratory of Membrane Biology; Chinese Academy of Sciences (CAS) - Institute for Stem Cell and Regeneration; Chinese Academy of Sciences (CAS) - University of Chinese Academy of Sciences

Dongyuan Ma

Chinese Academy of Sciences (CAS) - State Key Laboratory of Membrane Biology

Young-Ki Bae

National Cancer Center - Comparative Biomedical Research Branch

Guixian Liang

Chinese Academy of Sciences (CAS) - State Key Laboratory of Membrane Biology

Yifan Zhang

Chinese Academy of Sciences (CAS), Kunming Institute of Zoology, State Key Laboratory of Membrane Biology; Chinese Academy of Sciences (CAS) - Institute for Stem Cell and Regeneration; Chinese Academy of Sciences (CAS) - University of Chinese Academy of Sciences

Jung-Hwa Choi

Chungnam National University - Department of Biology

Cheol-Hee Kim

Chungnam National University - Department of Biology

Lu Wang

Peking Union Medical College, Chinese Academy of Medical Sciences, Institute of Hematology and Blood Diseases Hospital, State Key Laboratory of Experimental Hematology

Feng Liu

Chinese Academy of Sciences (CAS), Kunming Institute of Zoology, State Key Laboratory of Membrane Biology; Chinese Academy of Sciences (CAS) - Institute for Stem Cell and Regeneration; Chinese Academy of Sciences (CAS) - University of Chinese Academy of Sciences

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Abstract

Cell transplantation into immunodeficient recipients is a widely-used approach to study stem cell and cancer biology; however, studying cell states post transplantation in vivo is inconvenient in mammals. Here, we generated a zebrafish foxn1/Casper mutant that is transparent and immunodeficient with deficient T cells. By employing the mutant for hematopoietic stem cell transplantation (HSCT), we could achieve nonconditioned transplantation. Meanwhile, we found that fetal HSCs from 3 days post fertilization produce a better transplant outcome in adult recipients, compared to adult HSCs. Furthermore, adult HSCs from the newly generated Tg (runx1:en-GFP) line have higher engraftment efficiency than that from the Tg (CD41:GFP) line. In addition to HSCT, foxn1/Casper mutant is feasible to allogeneic leukemia and muscle cell transplantation, as well as xenogenic medaka HSCT. Collectively, foxn1/Casper mutant permits the nonconditioned engraftment of multiple cell types with superiority in imaging and characterization of transplanted cells at single cell resolution in vivo.

Keywords: foxn1/Casper mutant, Cell transplantation, Hematopoietic stem cells, Xenograft, Zebrafish

Suggested Citation

Gao, Shuai and Lv, Peng and Ma, Dongyuan and Bae, Young-Ki and Liang, Guixian and Zhang, Yifan and Choi, Jung-Hwa and Kim, Cheol-Hee and Wang, Lu and Liu, Feng, Generation of foxn1/ Casper Mutant Zebrafish for Allograft and Xenograft of Normal and Malignant Cells (August 20, 2019). CELL-REPORTS-D-19-02957. Available at SSRN: https://ssrn.com/abstract=3439669 or http://dx.doi.org/10.2139/ssrn.3439669
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Shuai Gao

Chinese Academy of Sciences (CAS) - State Key Laboratory of Membrane Biology

Beijing
China

Peng Lv

Chinese Academy of Sciences (CAS), Kunming Institute of Zoology, State Key Laboratory of Membrane Biology

Beijing
China

Chinese Academy of Sciences (CAS) - Institute for Stem Cell and Regeneration

Beijing
China

Chinese Academy of Sciences (CAS) - University of Chinese Academy of Sciences

Building 7, NO. 80 Zhongguancun Road
Beijing, Beijing 100190
China

Dongyuan Ma

Chinese Academy of Sciences (CAS) - State Key Laboratory of Membrane Biology

Beijing
China

Young-Ki Bae

National Cancer Center - Comparative Biomedical Research Branch

Korea, Republic of (South Korea)

Guixian Liang

Chinese Academy of Sciences (CAS) - State Key Laboratory of Membrane Biology

Beijing
China

Yifan Zhang

Chinese Academy of Sciences (CAS), Kunming Institute of Zoology, State Key Laboratory of Membrane Biology

Beijing
China

Chinese Academy of Sciences (CAS) - Institute for Stem Cell and Regeneration

Beijing
China

Chinese Academy of Sciences (CAS) - University of Chinese Academy of Sciences

Building 7, NO. 80 Zhongguancun Road
Beijing, Beijing 100190
China

Jung-Hwa Choi

Chungnam National University - Department of Biology

Korea, Republic of (South Korea)

Cheol-Hee Kim

Chungnam National University - Department of Biology

Korea, Republic of (South Korea)

Lu Wang

Peking Union Medical College, Chinese Academy of Medical Sciences, Institute of Hematology and Blood Diseases Hospital, State Key Laboratory of Experimental Hematology

NO. 9, Dongdan Santiao
Beijing
China

Feng Liu (Contact Author)

Chinese Academy of Sciences (CAS), Kunming Institute of Zoology, State Key Laboratory of Membrane Biology ( email )

Beijing
China

Chinese Academy of Sciences (CAS) - Institute for Stem Cell and Regeneration ( email )

Beijing
China

Chinese Academy of Sciences (CAS) - University of Chinese Academy of Sciences ( email )

Building 7, NO. 80 Zhongguancun Road
Beijing, Beijing 100190
China

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