RNA Structural Dynamics Regulates Early Embryogenesis Through Controlling Transcriptome Fate and Function
64 Pages Posted: 23 Aug 2019 Sneak Peek Status: Under ReviewMore...
Early embryogenesis in vertebrates is initially directed by maternal RNAs and proteins, yet the mechanisms controlling this program remain largely unknown. Recent transcriptome-wide studies on RNA structure have revealed its pervasive and crucial roles in RNA processing and functions. Here we established the first global map of four nucleotide-based mRNA structures during zebrafish early embryogenesis. Strikingly, we observed RNA structurally variable regions are enriched in the 3’ UTR, and contain cis-regulatory elements important for maternal-to-zygotic transition (MZT). We found that the RNA binding protein Elavl1a stabilized maternal mRNAs by binding to the cis-elements. Conversely, RNA structure formation suppressed Elavl1a’s binding leading to the decay of its maternal targets. Overall, our findings revealed a broad and fundamental role of RNA structure-based regulation in vertebrate early embryogenesis.
Keywords: RNA structure, zebrafish early embryogenesis, structure-based regulome, Elavl1a
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