header

The Effect of a Vascularized Network and Hierarchical Topographical Cue Using a Cell-Electrospinning/3D Printing Process on Inducing Myoblast-Alignment and Maturation

32 Pages Posted: 27 Aug 2019 First Look: Accepted

See all articles by Miji Yeo

Miji Yeo

Sungkyunkwan University - Department of Biomechatronic Engineering

GeunHyung Kim

Sungkyunkwan University - Department of Biomechatronic Engineering

Abstract

The human skeletal muscle is composed of intricate anatomical structures, including uniaxially arranged myotubes and widely distributed blood capillaries. In this regard, vascularization is an essential part of the successful development of an engineered skeletal muscle tissue to restore its function and physiological activities. In this paper, we propose a method to obtain an engineered vascularized skeletal muscle structure developed using cell-electrospinning and 3D bioprinting. To elaborate, on the surface of mechanical supporters (polycaprolactone and collagen struts) with a topographical cue, human umbilical vein endothelial cells (HUVECs)-laden alginate bioink was uniaxially electrospun. The electrospun HUVECs showed high cell viability (90%), homogeneous cell distribution, and efficient rearrangement into vessels. Furthermore, the myoblasts (C2C12 cells), which were seeded on the vascularized structure (HUVECs-laden fibers), were co-cultured to facilitate myoblast regeneration. As a result, the scaffold that included myoblasts and HUVECs represented a high degree of the myosin heavy chain with striated patterns and enhanced myogenic-specific gene expressions (MyoD, troponin T, and myogenin) as compared to the scaffold that included only myoblasts.

Keywords: cell-electrospinning, HUVECs, topographical cue, vascularization, scaffold, muscle

Suggested Citation

Yeo, Miji and Kim, GeunHyung, The Effect of a Vascularized Network and Hierarchical Topographical Cue Using a Cell-Electrospinning/3D Printing Process on Inducing Myoblast-Alignment and Maturation. Available at SSRN: https://ssrn.com/abstract=3441967 or http://dx.doi.org/10.2139/ssrn.3441967

Miji Yeo (Contact Author)

Sungkyunkwan University - Department of Biomechatronic Engineering

Korea, Republic of (South Korea)

GeunHyung Kim

Sungkyunkwan University - Department of Biomechatronic Engineering ( email )

Korea, Republic of (South Korea)

Here is the Coronavirus
related research on SSRN

Paper statistics

Abstract Views
199
Downloads
19