Tracking Dynamics of Spontaneous Tumours in Mice Using Photon Counting Computed Tomography
47 Pages Posted: 30 Aug 2019 Sneak Peek Status: PublishedMore...
Computed Tomography is a powerful medical imaging modality for longitudinal studies in cancer to follow neoplasia progression and evaluate anticancer therapies. Here, we report the generation of a new Photon Counting micro-Computed Tomography (PC-CT) method based on hybrid pixel detector with enhanced sensitivity and precision of tumour imaging. We then applied PC-CT for longitudinal imaging in a clinically relevant liver cancer model, the Alb-R26Met mice, and found a remarkable heterogeneity in the dynamics for tumours at the initiation phases. Instead, the growth curve of evolving tumours exhibited a comparable exponential growth, with a constant doubling time. Furthermore, longitudinal PC-CT imaging in mice treated with a combination of MEK and BCL-XL inhibitors revealed a drastic tumour regression accompanied by a striking remodelling of macrophages in the tumour microenvironment. Thus, PC-CT is a powerful system to detect cancer initiation and progression, and to monitor its evolution during treatment.
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