PLGA-Collagen-ECM Hybrid Meshes Mimicking Stepwise Osteogenesis and Their Influence on the Osteogenic Differentiation of hMSCs
25 Pages Posted: 13 Sep 2019
Extracellular matrices (ECMs) are dynamically altered and remodeled during tissue development. How the dynamic remodeling of ECMs affects stem cell functions remains poorly understood due to the difficulty of obtaining biomimetic ECMs. In this study, stepwise osteogenesis-mimicking ECM-deposited poly (lactic-coglycolic acid) (PLGA)-collagen hybrid meshes were prepared by culturing human mesenchymal stem cells (hMSCs) in PLGA-collagen hybrid meshes and controlling the stages of the osteogenesis of the hMSCs. Three types of hybrid meshes mimicking the ECMs of stem cell-stage hMSCs (SC-ECM scaffold) and the early stage (EO-ECM scaffold) and late stage (LO-ECM scaffold) of osteogenesis were prepared by controlling the osteogenic differentiation of hMSCs in the PLGA-collagen meshes at the stem cell stage and the early and late stages of osteogenesis. The stepwise osteogenesis-mimicking PLGA-collagen-ECM hybrid meshes showed different ECM compositions associated with the stage of osteogenesis, and their influence on the osteogenic differentiation of hMSCs differed. EO-ECM scaffolds increased and LO-ECM scaffolds moderately promoted the osteogenic differentiation of hMSCs. However, SC-ECM scaffolds showed an inhibitory effect. The novel PLGA-collagen-ECM hybrid meshes will provide useful tools for stem cell culture and tissue engineering.
Keywords: dynamically changed extracellular matrices, human mesenchymal stem cells, stepwise osteogenesis-mimicking, decellularization, PLGA-collagen-ECMs hybrid meshes
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