Sex-Specific Disruption of Distinct mPFC Inhibitory Neurons in Spared-Nerve Injury Model of Neuropathic Pain
46 Pages Posted: 16 Sep 2019 Sneak Peek Status: Under ReviewMore...
The medial prefrontal cortex (mPFC) modulates a broad range of behaviors including responses to noxious stimuli. While various pain modalities alter mPFC function, our understanding of changes to specific cell-types underlying pain-induced mPFC dysfunction remains incomplete. Proper activity of cortical GABAergic interneurons is essential for normal circuit function. We find that nerve injury increases excitability of layer 5 parvalbumin-expressing GABAergic neurons in the prelimbic (PL) region of mPFC from male, but not female mice. Conversely, nerve injury dampens excitability in somatostatin-expressing GABAergic neurons in layer 2/3 of PL; however, effects are differential between males and females. Nerve injury had minimal effect on spontaneous excitatory post-synaptic currents (sEPSCs) in parvalbumin-expressing neurons, but reduced frequency of sEPSCs in layer 2/3 somatostatin-expressing neurons in females. Our findings provide key insight into how nerve injury drives maladaptive and sex-specific alterations to GABAergic circuits in cortical regions implicated in affective disorders comorbid with chronic pain.
Keywords: Nerve injury, Pain, GABAergic neurons, Parvalbumin, Somatostatin, Slice Electrophysiology, Medial Prefrontal Cortex
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