header

In Vitro Measurement of the Chemical Changes Occurring within Β-Tricalcium Phosphate Bone Graft Substitutes

72 Pages Posted: 20 Sep 2019 First Look: Accepted

See all articles by Yassine Maazouz

Yassine Maazouz

RMS Foundation

Iris Rentsch

RMS Foundation

Bin Lu

RMS Foundation

Bastien Le Gars Santoni

RMS Foundation

Nicola Doebelin

RMS Foundation

Marc Bohner

RMS Foundation

Abstract

Several mechanisms proposed to explain the osteoinductive potential of calcium phosphates involve surface mineralization ("bioactivity") and mention the occurrence of concentration gradients between the inner and the outer part of the implanted material. Determining the evolution of the local chemical environment occurring inside the pores of an implanted bone graft substitute (BGS) is therefore highly relevant. A quantitative and fast method was developed to measure the chemical changes occurring within the pores of β-Tricalcium Phosphate (β-TCP) granules incubated in a simulated body fluid. A factorial design of experiment was used to test the effect of particle size, specific surface area, microporosity, and purity of the β-TCP granules. Large pH, calcium and phosphate concentration changes were observed inside the BGS and lasted for several days. The kinetics and magnitude of these changes (up to 2 pH units) largely depended on the processing and properties of the granules. Interestingly, processing parameters that increased the kinetics and magnitude of the local chemical changes are parameters considered to favor calcium phosphate osteoinduction, suggesting that the model might be useful to predict the osteoinductive potential of BGSs.

Keywords: Calcium phosphate, Calcium, Phosphate, Local pH, Bioactivity, Osteoinductivity, Ectopic bone formation, Heterotopic bone formation, Bone graft substitute, Beta-tricalcium phosphate

Suggested Citation

Maazouz, Yassine and Rentsch, Iris and Lu, Bin and Santoni, Bastien Le Gars and Doebelin, Nicola and Bohner, Marc, In Vitro Measurement of the Chemical Changes Occurring within Β-Tricalcium Phosphate Bone Graft Substitutes. Available at SSRN: https://ssrn.com/abstract=3454942

Yassine Maazouz

RMS Foundation ( email )

Bischmattstrasse, 12
Bettlach, Solothurn 2544
Switzerland

Iris Rentsch

RMS Foundation ( email )

Bischmattstrasse, 12
Bettlach, Solothurn 2544
Switzerland

Bin Lu

RMS Foundation ( email )

Bischmattstrasse, 12
Bettlach, Solothurn 2544
Switzerland

Bastien Le Gars Santoni

RMS Foundation

Bischmattstrasse, 12
Bettlach, Solothurn 2544
Switzerland

Nicola Doebelin

RMS Foundation ( email )

Bischmattstrasse, 12
Bettlach, Solothurn 2544
Switzerland

Marc Bohner (Contact Author)

RMS Foundation ( email )

Bischmattstrasse, 12
Bettlach, Solothurn 2544
Switzerland
+41 32 6442040 (Phone)
+41 32 644 2076 (Fax)

Here is the Coronavirus
related research on SSRN

Paper statistics

Abstract Views
157
Downloads
35