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DNA Content Contributes to Nuclear Size Control in Xenopus

64 Pages Posted: 1 Oct 2019 Sneak Peek Status: Review Complete

See all articles by Hiroko Heijo

Hiroko Heijo

Yamaguchi University - Evolutionary Cell Biology Laboratory

Sora Shimogama

Yamaguchi University - Evolutionary Cell Biology Laboratory

Shuichi Nakano

Yamaguchi University - Evolutionary Cell Biology Laboratory

Anna Miyata

Yamaguchi University - Evolutionary Cell Biology Laboratory

Yasuhiro Iwao

Yamaguchi University - Laboratory of Molecular Developmental Biology

Yuki Hara

Yamaguchi University - Evolutionary Cell Biology Laboratory

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Abstract

Cells adapt to drastic changes in genome quantity during evolution and cell division by adjusting the nuclear size to exert genomic functions. However, the mechanism by which DNA content within the nucleus contributes to controlling the nuclear size, remains unclear. Here, we experimentally evaluated the effects of DNA content by utilizing cell-free Xenopus egg extracts and imaging of in vivo embryos. Upon manipulation of DNA content while maintaining cytoplasmic effects as constant, both plateau size and expansion speed of the nucleus correlated highly with DNA content. We also found an increase in the amount of membranes on the nuclear surface when DNA content was low and alterations in the nuclear expansion dynamics after converting the chromatin structures. These results demonstrate a novel model in which the physical properties of the chromatin, rather than the coding sequences themselves, contribute to nuclear expansion by generating forces that expand the nucleus outwardly.

Keywords: Nucleus, Nuclear size, DNA content, Genome size, Xenopus, in vitro cell-free reconstruction, in vivo imaging, organelle size, size scaling

Suggested Citation

Heijo, Hiroko and Shimogama, Sora and Nakano, Shuichi and Miyata, Anna and Iwao, Yasuhiro and Hara, Yuki, DNA Content Contributes to Nuclear Size Control in Xenopus (September 30, 2019). Available at SSRN: https://ssrn.com/abstract=3461734 or http://dx.doi.org/10.2139/ssrn.3461734
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Hiroko Heijo

Yamaguchi University - Evolutionary Cell Biology Laboratory

Japan

Sora Shimogama

Yamaguchi University - Evolutionary Cell Biology Laboratory

Japan

Shuichi Nakano

Yamaguchi University - Evolutionary Cell Biology Laboratory

Japan

Anna Miyata

Yamaguchi University - Evolutionary Cell Biology Laboratory

Japan

Yasuhiro Iwao

Yamaguchi University - Laboratory of Molecular Developmental Biology

Japan

Yuki Hara (Contact Author)

Yamaguchi University - Evolutionary Cell Biology Laboratory

Japan

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