Determination of Chlorpheniramine Maleate in the Presence of Tartrazine in Tablets Using High Performance Thin Layer Chromatography

6 Pages Posted: 21 Oct 2019 Last revised: 6 Dec 2019

See all articles by F. Annuryanti

F. Annuryanti

Airlangga University - Faculty of Pharmacy, Airlangga University

Asri Darmawati

Airlangga University - Faculty of Pharmacy, Airlangga University

R. Primaharinastiti

Faculty of Pharmacy, Airlangga University

M. Kusoiri

Faculty of Pharmacy, Airlangga University

Date Written: 2018

Abstract

Tartrazine is a colouring substance suspected to interfere with the determination of chlorpheniramine maleate (CPM) in tablet analyzed using USP39/NF34 method. The purpose of this study, therefore, was to separate tartrazine using High Performance Thin Layer Chromatography (HPTLC), and five microliters of samples with standard solution were spotted on the HPTLC plate of silica gel 60 F254. Furthermore, the result showed the ability of a mixture of isopropanol and NH4OH (24:6 v/v) as a mobile phase to separate tartrazine and maleic acid from chlorpheniramine, with Rs value of > 1.5. Meanwhile, the HPTLC validation method used were as follows: linearity of CPM in the range concentration of 400-5000 ppm was 0.998, while the accuracy and precision was 99.93 % and 3.50%, respectively. In addition, drug determination in the registered tablets was conducted using the proposed technique, which showed that concentrations in samples met the specifications as stated in USP39/NF34 monography.

Keywords: chlorpheniramine maleate, tartrazine, HPTLC

Suggested Citation

Annuryanti, F. and Darmawati, Asri and Primaharinastiti, R. and Kusoiri, M., Determination of Chlorpheniramine Maleate in the Presence of Tartrazine in Tablets Using High Performance Thin Layer Chromatography (2018). Proceedings of International Conference on Applied Pharmaceutical Sciences (ICoAPS) 2018. Available at SSRN: https://ssrn.com/abstract=3467488 or http://dx.doi.org/10.2139/ssrn.3467488

F. Annuryanti

Airlangga University - Faculty of Pharmacy, Airlangga University

FF UNAIR Kampus C UNAIR
Surabaya, East Java 60115
Indonesia

HOME PAGE: http://ff.unair.ac.id/

Asri Darmawati (Contact Author)

Airlangga University - Faculty of Pharmacy, Airlangga University ( email )

FF UNAIR Kampus C UNAIR
Surabaya, East Java 60115
Indonesia
+620315933150 (Phone)
+620315935249 (Fax)

HOME PAGE: http://www.ff.unair.ac.id

R. Primaharinastiti

Faculty of Pharmacy, Airlangga University

FF UNAIR Kampus C UNAIR
Surabaya, East Java 60115
Indonesia

HOME PAGE: http://ff.unair.ac.id/

M. Kusoiri

Faculty of Pharmacy, Airlangga University

FF UNAIR Kampus C UNAIR
Surabaya, East Java 60115
Indonesia

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