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SpiCee: A Genetic Tool for Subcellular and Cell-Specific Calcium Manipulation

39 Pages Posted: 16 Oct 2019 Sneak Peek Status: Review Complete

See all articles by Oriol Ros

Oriol Ros

Sorbonne Université - Institut de la Vision

Yvrick Zagar

Sorbonne Université - Institut de la Vision

Karine Loulier

INSERM, Institut des Neurosciences de Montpellier

Sandrine Couvet

Sorbonne Université - Institut de la Vision

Alain Aghaie

Sorbonne Université - Institut Pasteur

Fiona Roche

Sorbonne Université - Institut de la Vision

Sarah Baudet

Sorbonne Université - Institut de la Vision

Alice Louail

Sorbonne Université - Institut de la Vision

Christine Petit

Sorbonne Université - Institut Pasteur

Yves Mechulam

Ecole Polytechnique, Palaiseau - Laboratoire de Biochimie

Xavier Nicol

Sorbonne Université - Institut de la Vision

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Abstract

Calcium is a second messenger crucial to a myriad of cellular processes ranging from regulation of metabolism and cell survival to vesicle release and motility. Current strategies to directly manipulate endogenous calcium signals lack cellular and subcellular specificity. We developed SpiCee, a versatile and genetically-encoded chelator combining low and high affinity sites for calcium. This scavenger enables altering endogenous calcium signaling and functions in single cells in vitro and in vivo with biochemically-controlled subcellular resolution. SpiCee paves the way to investigate local calcium signaling in vivo and directly manipulate this second messenger for therapeutic use.

Keywords: calcium buffer, subcellular compartment, single cell pharmacology, axon guidance, neuronal migration

Suggested Citation

Ros, Oriol and Zagar, Yvrick and Loulier, Karine and Couvet, Sandrine and Aghaie, Alain and Roche, Fiona and Baudet, Sarah and Louail, Alice and Petit, Christine and Mechulam, Yves and Nicol, Xavier, SpiCee: A Genetic Tool for Subcellular and Cell-Specific Calcium Manipulation (October 16, 2019). CELL-REPORTS-D-19-04061. Available at SSRN: https://ssrn.com/abstract=3470393 or http://dx.doi.org/10.2139/ssrn.3470393
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Oriol Ros

Sorbonne Université - Institut de la Vision ( email )

17 rue Moreau
Paris, 75012
France

Yvrick Zagar

Sorbonne Université - Institut de la Vision ( email )

17 rue Moreau
Paris, 75012
France

Karine Loulier

INSERM, Institut des Neurosciences de Montpellier ( email )

Hôpital Saint Eloi, INM, Inserm U1051
80 avenue Augustin Fliche
Montpellier, 34000
France

Sandrine Couvet

Sorbonne Université - Institut de la Vision ( email )

17 rue Moreau
Paris, 75012
France

Alain Aghaie

Sorbonne Université - Institut Pasteur

France

Fiona Roche

Sorbonne Université - Institut de la Vision ( email )

17 rue Moreau
Paris, 75012
France

Sarah Baudet

Sorbonne Université - Institut de la Vision ( email )

17 rue Moreau
Paris, 75012
France

Alice Louail

Sorbonne Université - Institut de la Vision ( email )

17 rue Moreau
Paris, 75012
France

Christine Petit

Sorbonne Université - Institut Pasteur

France

Yves Mechulam

Ecole Polytechnique, Palaiseau - Laboratoire de Biochimie ( email )

France

Xavier Nicol (Contact Author)

Sorbonne Université - Institut de la Vision ( email )

17 rue Moreau
Paris, 75012
France

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