Development and Validation of an HPLC Method for the Determination of p-methoxycinnamic Acid in Rabbit Plasma

6 Pages Posted: 31 Oct 2019 Last revised: 6 Dec 2019

See all articles by Dewi Isadiartuti

Dewi Isadiartuti

Faculty of Pharmacy, Airlangga University

I.K. Prihantini

Faculty of Pharmacy, Airlangga University

A.P. Agustina

Faculty of Pharmacy, Airlangga University

T. Aryani

Faculty of Pharmacy, Airlangga University

A. Syahrani

Faculty of Pharmacy, Airlangga University

Date Written: September 31, 2019

Abstract

p-methoxycinnamic acid (pMCA) is an active compound obtained from the hydrolysis of ethyl p- methoxycinnamate acid (EpMC). It is isolated from Kaempferia galanga Linn with anti-inflammatory and analgesic effects. A simple, rapid, accurate and selective high performance liquid chromatography method was developed to determine the p-methoxycinnamic acid consentrations in rabbit plasma using a C18 reversed-phase analytical column. Ethanol was used for protein precipitation to prepare plasma samples, with chromatographic separation performed using a ยต-Bondapak C18 (3.9 mm x 300 mm i.d). The mobile phase consists of methanol and water (30:70), with a flow rate 0.8 mL/min, maximum pressure of 300 bars and UV detection of 286 nm. The proposed method was validated by performing selectivity, linearity, accuracy, precision, LOD and LOQ for pMCA with no interfering peaks. Its overall analytical run time was 10 min, which is useful for in vivo studies of p-methoxycinnamic acid.

Keywords: pMCA, rabbit plasma, validation, HPLC method

Suggested Citation

Isadiartuti, Dewi and Prihantini, I.K. and Agustina, A.P. and Aryani, T. and Syahrani, A., Development and Validation of an HPLC Method for the Determination of p-methoxycinnamic Acid in Rabbit Plasma (September 31, 2019). Proceedings of International Conference on Applied Pharmaceutical Sciences (ICoAPS) 2018. Available at SSRN: https://ssrn.com/abstract=3478430 or http://dx.doi.org/10.2139/ssrn.3478430

Dewi Isadiartuti (Contact Author)

Faculty of Pharmacy, Airlangga University ( email )

FF UNAIR Kampus C UNAIR
Surabaya, East Java 60115
Indonesia
5033710 (Phone)
60115 (Fax)

HOME PAGE: http://ff.unair.ac.id/

I.K. Prihantini

Faculty of Pharmacy, Airlangga University

Jl. Darmawangsa Dalam 2
Surabaya, 60286
Indonesia
5033710 (Phone)
60115 (Fax)

HOME PAGE: http://ff.unair.ac.id/

A.P. Agustina

Faculty of Pharmacy, Airlangga University

Jl. Darmawangsa Dalam 2
Surabaya, 60286
Indonesia
5033710 (Phone)
60115 (Fax)

HOME PAGE: http://ff.unair.ac.id/

T. Aryani

Faculty of Pharmacy, Airlangga University

FF UNAIR Kampus C UNAIR
Surabaya, East Java 60115
Indonesia
5033710 (Phone)
60115 (Fax)

HOME PAGE: http://ff.unair.ac.id/

A. Syahrani

Faculty of Pharmacy, Airlangga University

Jl. Darmawangsa Dalam 2
Surabaya, 60286
Indonesia
5033710 (Phone)
60115 (Fax)

HOME PAGE: http://ff.unair.ac.id/

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