puc-header

A Bacterial Effector Protein Hijacks Plant Metabolism to Support Bacterial Nutrition

50 Pages Posted: 4 Nov 2019 Sneak Peek Status: Review Complete

See all articles by Liu Xian

Liu Xian

Chinese Academy of Sciences (CAS) - Shanghai Center for Plant Stress Biology

Gang Yu

Chinese Academy of Sciences (CAS) - Shanghai Center for Plant Stress Biology

Yali Wei

Chinese Academy of Sciences (CAS) - Shanghai Center for Plant Stress Biology

Yansha Li

Chinese Academy of Sciences (CAS) - Shanghai Center for Plant Stress Biology

Hao Xue

Chinese Academy of Sciences (CAS) - Shanghai Center for Plant Stress Biology

Rafael Morcillo

Chinese Academy of Sciences (CAS) - Shanghai Center for Plant Stress Biology

Jose Rufian

Chinese Academy of Sciences (CAS) - Shanghai Center for Plant Stress Biology

Alberto Macho

Chinese Academy of Sciences (CAS) - Shanghai Center for Plant Stress Biology

More...

Abstract

Bacterial diseases are a threat to food security. Most bacterial pathogens inject effector proteins inside plant cells, using a type-III secretion system, to suppress plant immunity. However, whether and how effector proteins co-opt plant metabolism to produce nutrients that support extensive bacterial replication is not understood. In this work, we found that Ralstonia solanacearum, the causal agent of bacterial wilt disease, secretes an effector protein, named RipI, which interacts with host glutamate decarboxylases (GADs) inside plant cells. GADs catalyse the biosynthesis of GABA, and are activated by calmodulin. RipI promotes the interaction of GADs with calmodulin, enhancing the production of GABA. Unlike other pathogens, Ralstonia is able to replicate efficiently using GABA as a nutrient, and requires both RipI and plant GABA to achieve a successful infection. This reveals a pathogenic strategy to hijack plant metabolism for the biosynthesis of nutrients to support microbial growth during plant colonization.

Keywords: Ralstonia, effector, GABA, virulence, Nutrition, metabolism, calmodulin, GAD

Suggested Citation

Xian, Liu and Yu, Gang and Wei, Yali and Li, Yansha and Xue, Hao and Morcillo, Rafael and Rufian, Jose and Macho, Alberto, A Bacterial Effector Protein Hijacks Plant Metabolism to Support Bacterial Nutrition (November 2, 2019). CELL-HOST-MICROBE-D-19-00886. Available at SSRN: https://ssrn.com/abstract=3479448 or http://dx.doi.org/10.2139/ssrn.3479448
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Liu Xian

Chinese Academy of Sciences (CAS) - Shanghai Center for Plant Stress Biology

China

Gang Yu

Chinese Academy of Sciences (CAS) - Shanghai Center for Plant Stress Biology

China

Yali Wei

Chinese Academy of Sciences (CAS) - Shanghai Center for Plant Stress Biology

China

Yansha Li

Chinese Academy of Sciences (CAS) - Shanghai Center for Plant Stress Biology

China

Hao Xue

Chinese Academy of Sciences (CAS) - Shanghai Center for Plant Stress Biology

China

Rafael Morcillo

Chinese Academy of Sciences (CAS) - Shanghai Center for Plant Stress Biology

China

Jose Rufian

Chinese Academy of Sciences (CAS) - Shanghai Center for Plant Stress Biology

China

Alberto Macho (Contact Author)

Chinese Academy of Sciences (CAS) - Shanghai Center for Plant Stress Biology ( email )

China

Click here to go to Cell.com

Paper statistics

Abstract Views
790
Downloads
21