puc-header

Self-Regulation of Autophagy Genes Expression by Atg8a and Its Interacting Partners YL-1, Sequoia and Sir2 In  Drosophila

33 Pages Posted: 4 Dec 2019 Publication Status: Review Complete

See all articles by Anne-Claire Jacomin

Anne-Claire Jacomin

University of Warwick - School of Life Sciences and Mathematics Institute

Stavroula Petridi

University of Warwick - School of Life Sciences and Mathematics Institute

Marisa Di Monaco

University of Warwick - School of Life Sciences and Mathematics Institute

Zambarlal Bhujabal

University of Tromsø - The Arctic University of Norway - Molecular Cancer Research Group

Ashish Jain

University of Tromsø - The Arctic University of Norway - Molecular Cancer Research Group

Nitha Mulakkal

University of Warwick - School of Life Sciences and Mathematics Institute

Anthimi Palara

University of Warwick - School of Life Sciences and Mathematics Institute

Emma L. Powell

University of Warwick - School of Life Sciences and Mathematics Institute

Bonita Chung

University of Warwick - School of Life Sciences and Mathematics Institute

Cleidi Zampronio

University of Warwick - School of Life Sciences and Mathematics Institute

Alexandra Jones

University of Warwick - School of Life Sciences and Mathematics Institute

Alexander Cameron

University of Warwick - School of Life Sciences and Mathematics Institute

Terje Johansen

University of Tromsø - The Arctic University of Norway - Molecular Cancer Research Group

Ioannis Nezis

University of Warwick - School of Life Sciences and Mathematics Institute

More...

Abstract

Autophagy is a process in which cytoplasmic material is degraded through the lysosomal pathway. One of the most studied autophagy-related proteins is LC3, which participates in autophagosome formation and autophagy cargo selection in the cytoplasm. Despite growing evidence that LC3 is enriched in the nucleus, little is known about the nuclear components it interacts with. Here we show that Drosophila Atg8a protein, homologous to mammalian LC3, interacts with the transcription factor Sequoia in a LIR-motif dependent manner. We show that Sequoia depletion induces autophagy in nutrient rich conditions through enhanced expression of autophagy genes. We show that Atg8a interacts with YL-1, a component of a nuclear acetyltransferase complex and that it is acetylated in nutrient rich conditions. We also show that Atg8a interacts with the deacetylase Sir2, which deacetylates Atg8a during starvation in order to activate autophagy. Our results suggest a mechanism of regulation of expression of autophagy genes by Atg8a, which is linked to its  acetylation status and its interaction with Sequoia,  YL-1 and Sir2.

Keywords: acetylation, autophagy, LIR motif, nucleus, transcription, LC3/Atg8

Suggested Citation

Jacomin, Anne-Claire and Petridi, Stavroula and Di Monaco, Marisa and Bhujabal, Zambarlal and Jain, Ashish and Mulakkal, Nitha and Palara, Anthimi and Powell, Emma L. and Chung, Bonita and Zampronio, Cleidi and Jones, Alexandra and Cameron, Alexander and Johansen, Terje and Nezis, Ioannis, Self-Regulation of Autophagy Genes Expression by Atg8a and Its Interacting Partners YL-1, Sequoia and Sir2 In  Drosophila (November 27, 2019). Available at SSRN: https://ssrn.com/abstract=3494305 or http://dx.doi.org/10.2139/ssrn.3494305
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Anne-Claire Jacomin

University of Warwick - School of Life Sciences and Mathematics Institute

United Kingdom

Stavroula Petridi

University of Warwick - School of Life Sciences and Mathematics Institute

United Kingdom

Marisa Di Monaco

University of Warwick - School of Life Sciences and Mathematics Institute

United Kingdom

Zambarlal Bhujabal

University of Tromsø - The Arctic University of Norway - Molecular Cancer Research Group

Norway

Ashish Jain

University of Tromsø - The Arctic University of Norway - Molecular Cancer Research Group

Norway

Nitha Mulakkal

University of Warwick - School of Life Sciences and Mathematics Institute

United Kingdom

Anthimi Palara

University of Warwick - School of Life Sciences and Mathematics Institute

United Kingdom

Emma L. Powell

University of Warwick - School of Life Sciences and Mathematics Institute

United Kingdom

Bonita Chung

University of Warwick - School of Life Sciences and Mathematics Institute

United Kingdom

Cleidi Zampronio

University of Warwick - School of Life Sciences and Mathematics Institute

United Kingdom

Alexandra Jones

University of Warwick - School of Life Sciences and Mathematics Institute

United Kingdom

Alexander Cameron

University of Warwick - School of Life Sciences and Mathematics Institute

United Kingdom

Terje Johansen

University of Tromsø - The Arctic University of Norway - Molecular Cancer Research Group

Norway

Ioannis Nezis (Contact Author)

University of Warwick - School of Life Sciences and Mathematics Institute ( email )

United Kingdom

Click here to go to Cell.com

Paper statistics

Abstract Views
466
Downloads
9
PlumX Metrics