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Microfluidics Synthesis of Injectable Angiogenic Microgels

56 Pages Posted: 6 Dec 2019 Sneak Peek Status: Review Complete

See all articles by Haoran Zhao

Haoran Zhao

Tsinghua University - Tsinghua-Berkeley Shenzhen Institute

Zitian Wang

Tsinghua University - Tsinghua-Berkeley Shenzhen Institute

Shengwei Jiang

Tsinghua University - Tsinghua-Berkeley Shenzhen Institute

Jiaqi Wang

Tsinghua University - Tsinghua-Berkeley Shenzhen Institute

Zhiwei Hu

Tsinghua University - Tsinghua-Berkeley Shenzhen Institute

Peter E. Lobie

Tsinghua University - Tsinghua-Berkeley Shenzhen Institute

Shaohua Ma

Tsinghua University - Tsinghua-Berkeley Shenzhen Institute

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Abstract

Implanting cell-laden microgels repairs soft tissue injuries, under the co-occurrence of tissue remodeling and angiogenesis in the engrafts. Engineering microgels using native-tissue derived materials, such as collagen, remains a challenge because they are mostly slow-gelling and not angiogenic. Herein, we invented a fast-gelling and angiogenic collagen scaffold interlocked by ultra-long DNA (over 100,000 nt) programmed with vascular endothelial growth factor (VEGF) aptamers. The DNA interlocking shortened the collagen gelation time by over 30-fold, to 40 seconds in surfactant-free microfluidics and modulated by the flow, but unaltered the viscoelasticity, biodegradability, and biochemical properties of native collagen. When encapsulated with cells, the angiogenic microgels remarkably improved wound healing and liver regeneration, compared with the non-angiogenic or acellular microgels. After cryopreservation, the cell-laden microgels were retrieved with over 80% cells viable and retained their therapeutic potential. Thereby, these angiogenic, fast-gelling and ultra-soft cell-laden microgels are promising candidates as injectable therapeutics in regenerative medicine.

Keywords: Microfluidics, microgels, collagen, ultra-long DNA, angiogenesis

Suggested Citation

Zhao, Haoran and Wang, Zitian and Jiang, Shengwei and Wang, Jiaqi and Hu, Zhiwei and Lobie, Peter E. and Ma, Shaohua, Microfluidics Synthesis of Injectable Angiogenic Microgels (December 3, 2019). Available at SSRN: https://ssrn.com/abstract=3497026 or http://dx.doi.org/10.2139/ssrn.3497026
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Haoran Zhao

Tsinghua University - Tsinghua-Berkeley Shenzhen Institute

Beijing, 100084
China

Zitian Wang

Tsinghua University - Tsinghua-Berkeley Shenzhen Institute

Beijing, 100084
China

Shengwei Jiang

Tsinghua University - Tsinghua-Berkeley Shenzhen Institute ( email )

Beijing, 100084
China

Jiaqi Wang

Tsinghua University - Tsinghua-Berkeley Shenzhen Institute

Beijing, 100084
China

Zhiwei Hu

Tsinghua University - Tsinghua-Berkeley Shenzhen Institute

Beijing, 100084
China

Peter E. Lobie

Tsinghua University - Tsinghua-Berkeley Shenzhen Institute ( email )

Beijing, 100084
China

Shaohua Ma (Contact Author)

Tsinghua University - Tsinghua-Berkeley Shenzhen Institute ( email )

Beijing, 100084
China

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