Characterization of Bioactive Compounds From Marine Red Algae Gracillaria Edulis
Posted: 4 Dec 2019
Date Written: December 3, 2019
The prevalence of diabetes mellitus (DM) is increasing worldwide due to lifestyle change associated with rapid urbanization. Natural bioactive compounds abundant in red algae can be utilised in drug leads and nutraceuticals. However, Sri Lankan marine algae are underexploited. The present study aimed to evaluate the hypoglycemic effect of methanol extract of G.edulis (Family: Gracilariaceae) using inhibitory activity of alpha glucosidase enzyme and glucose diffusion. The active fraction was subjected for GCMS analysis. De-polysaccharide methanol extract of G.edulis was sequentially partitioned with hexane, chloroform, ethyl acetate and In-vitro hypoglycemic potential was evaluated in crude methanol extract (ME), hexane (HF), chloroform (CF), ethyl acetate (EAF) and aqueous (AF) fractions. The fractions and the ME exhibited a dose-dependent inhibition of α-glucosidase enzyme, with EAF showing the highest inhibition of α-glucosidase enzyme (IC50: 87.92±1.62 µg/mL) compared to the standard acarbose (IC50:0.38±0.06 µg/mL). The hexane fraction with the highest IC50 value of 163.90±5.23 µg/mL reveled the weakest α-glucosidase inhibitory activity. Among the tested ME and the fractions, EAF exhibited maximum inhibition of glucose diffusion at 180 min with 38.15±1.11µg/mL glucose concentration in the external solution compared to the standard acarbose (22.79±0.47 µg/mL). Further, the active EAF of G. edulis was subjected to GC-MS analysis, and six compounds were identified. Among them, 1H-Indole-2-carboxylic acid,6-(4-ethoxyphenyl)-3-methyl-4-oxo-4,5,6,7-tetrahydro-isopropyl ester possess anti-diabetic activities while 2,5-dimethylhexane-2,5-dihydroperoxide was identified to have antioxidant properties. Therefore, the results of the study clearly showed the potential use of EAF of G.edulis to manage hyperglycaemia and its complications.
Keywords: gracillaria edulis, α-glucosidase, glucose diffusion, GCMS
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