Characterization of Bioactive Compounds From Marine Red Algae Gracillaria Edulis

Posted: 4 Dec 2019

See all articles by MDTL Gunathilaka

MDTL Gunathilaka

Department of Zoology, (Center for Instrumentation Facility & Center for Biotechnology), University of Sri Jayewardenepura,

DMIH Dissanayake

Department of Zoology, (Center for Instrumentation Facility & Center for Biotechnology), University of Sri Jayewardenepura

P Ranasinghe

Industrial Technology Institute, Habarawa Gardens, Malabe

KW Samarakoon

National Science and Technology Commission, Colombo

LDC Peiris

Department of Zoology, (Center for Instrumentation Facility & Center for Biotechnology), University of Sri Jayewardenepura

Date Written: December 3, 2019

Abstract

The prevalence of diabetes mellitus (DM) is increasing worldwide due to lifestyle change associated with rapid urbanization. Natural bioactive compounds abundant in red algae can be utilised in drug leads and nutraceuticals. However, Sri Lankan marine algae are underexploited. The present study aimed to evaluate the hypoglycemic effect of methanol extract of G.edulis (Family: Gracilariaceae) using inhibitory activity of alpha glucosidase enzyme and glucose diffusion. The active fraction was subjected for GCMS analysis. De-polysaccharide methanol extract of G.edulis was sequentially partitioned with hexane, chloroform, ethyl acetate and In-vitro hypoglycemic potential was evaluated in crude methanol extract (ME), hexane (HF), chloroform (CF), ethyl acetate (EAF) and aqueous (AF) fractions. The fractions and the ME exhibited a dose-dependent inhibition of α-glucosidase enzyme, with EAF showing the highest inhibition of α-glucosidase enzyme (IC50: 87.92±1.62 µg/mL) compared to the standard acarbose (IC50:0.38±0.06 µg/mL). The hexane fraction with the highest IC50 value of 163.90±5.23 µg/mL reveled the weakest α-glucosidase inhibitory activity. Among the tested ME and the fractions, EAF exhibited maximum inhibition of glucose diffusion at 180 min with 38.15±1.11µg/mL glucose concentration in the external solution compared to the standard acarbose (22.79±0.47 µg/mL). Further, the active EAF of G. edulis was subjected to GC-MS analysis, and six compounds were identified. Among them, 1H-Indole-2-carboxylic acid,6-(4-ethoxyphenyl)-3-methyl-4-oxo-4,5,6,7-tetrahydro-isopropyl ester possess anti-diabetic activities while 2,5-dimethylhexane-2,5-dihydroperoxide was identified to have antioxidant properties. Therefore, the results of the study clearly showed the potential use of EAF of G.edulis to manage hyperglycaemia and its complications.

Keywords: gracillaria edulis, α-glucosidase, glucose diffusion, GCMS

Suggested Citation

Gunathilaka, MDTL and Dissanayake, DMIH and Ranasinghe, P and Samarakoon, KW and Peiris, LDC, Characterization of Bioactive Compounds From Marine Red Algae Gracillaria Edulis (December 3, 2019). Proceedings of the 6th International Conference on Multidisciplinary Approaches (iCMA) 2019 | Faculty of Graduate Studies, University of Sri Jayewardenepura. Available at SSRN: https://ssrn.com/abstract=3497293

MDTL Gunathilaka (Contact Author)

Department of Zoology, (Center for Instrumentation Facility & Center for Biotechnology), University of Sri Jayewardenepura, ( email )

Sri Lanka

DMIH Dissanayake

Department of Zoology, (Center for Instrumentation Facility & Center for Biotechnology), University of Sri Jayewardenepura ( email )

Sri Lanka

P Ranasinghe

Industrial Technology Institute, Habarawa Gardens, Malabe ( email )

Habarawa Gardens, Malabe, Sri Lanka
Sri Lanka

KW Samarakoon

National Science and Technology Commission, Colombo ( email )

Colombo
Sri Lanka

LDC Peiris

Department of Zoology, (Center for Instrumentation Facility & Center for Biotechnology), University of Sri Jayewardenepura ( email )

Sri Lanka

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