Phytochemical Profile of Main Antioxidants in Different Fractions of Chnoospora Minima and Gracillaria Edulis

Posted: 5 Dec 2019

See all articles by MDTL Gunathilaka

MDTL Gunathilaka

Department of Zoology, (Center for Instrumentation Facility & Center for Biotechnology), University of Sri Jayewardenepura,

WLR Keerthirathna

Faculty of Graduate Studies, University of Sri Jayewardenepura

P Ranasinghe

Industrial Technology Institute, Habarawa Gardens, Malabe

KW Samarakoon

National Science and Technology Commission, Colombo

LDC Peiris

Department of Zoology, (Center for Instrumentation Facility & Center for Biotechnology), University of Sri Jayewardenepura

Date Written: December 3, 2019

Abstract

Natural bioactive compounds are abundant in seaweeds with potentials therapeutic activities. As Sri Lankan marine algae are underexploited, here we aimed to investigate the total phenolic, flavonoid contents and antioxidant activities of the methanol extract of Chnoospora minima and Gracillaria edulis and its fractions. De-polysaccharide methanol extracts of C.minima and G.edulis were partitioned with hexane, chloroform, ethyl acetate and phytochemicals, antioxidant activities were determined in the crude methanol extract (ME), hexane (HF), chloroform (CF), ethyl acetate (EAF) and aqueous (AF) fractions. Total phenolic (TPC) and flavonoid contents (TFC) were determined using Folin–Ciocalteu and aluminium chloride methods. Total alkaloid content was determined using the standard method. Antioxidant potentials were determined using ferric reducing antioxidant power (FRAP), ferrous iron chelating activity (FICA), 2, 2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2,-2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. The highest TPC and TFC were recorded in the ME (59.01± 1.86 mg GAE/g) and EAF (5.14±0.43 mg QE/g) of C.minima whereas the highest alkaloid content was reported in ME (7.17±0.06 mg PE/g) of G.edulis. The highest DPPH activity was recorded in ME (IC50: 0.48± 0.01 mg/ml) of C.minima compared to the standard trolox (IC50: 0.011 mg/ml). However, most potent ABTS scavenging activity was detected in EAF (IC50: 0.06±0.001 mg/ml) of C.minima while the lowest was recorded in ME (IC50:0.56 ± 0.01 mg/ml) of G.edulis compared to the standard Trolox (IC50: 0.008 mg/ml). Compared to the chelation capacity, the highest FICA was found in EAF (IC50: 0.38±0.01 mg/ml) of C.minima while the lowest was in the ME (IC50: 9.23 ± 0.19 mg/ml) of G.edulis compared to the standard EDTA (IC50-0.019 mg/ml). Similarly, EAF (20.34±1.72 mg TE/g) of C.minima showed highest reducing power, whereas in ME of G.edulis recorded the lowest reducing power (0.26 ± 0.03 (mg TE/g). In conclusion, ME and EAF of C.minima possess marked antioxidant activities, which can be used for management of oxidative stress-associated chronic diseases.

Keywords: gracillaria edulis, choonospora minima, phytochemicals, antioxidants

Suggested Citation

Gunathilaka, MDTL and Keerthirathna, WLR and Ranasinghe, P and Samarakoon, KW and Peiris, LDC, Phytochemical Profile of Main Antioxidants in Different Fractions of Chnoospora Minima and Gracillaria Edulis (December 3, 2019). Proceedings of the 6th International Conference on Multidisciplinary Approaches (iCMA) 2019 | Faculty of Graduate Studies, University of Sri Jayewardenepura. Available at SSRN: https://ssrn.com/abstract=3497395

MDTL Gunathilaka (Contact Author)

Department of Zoology, (Center for Instrumentation Facility & Center for Biotechnology), University of Sri Jayewardenepura, ( email )

Sri Lanka

WLR Keerthirathna

Faculty of Graduate Studies, University of Sri Jayewardenepura ( email )

Sri Lanka

P Ranasinghe

Industrial Technology Institute, Habarawa Gardens, Malabe ( email )

Habarawa Gardens, Malabe, Sri Lanka
Sri Lanka

KW Samarakoon

National Science and Technology Commission, Colombo ( email )

Colombo
Sri Lanka

LDC Peiris

Department of Zoology, (Center for Instrumentation Facility & Center for Biotechnology), University of Sri Jayewardenepura ( email )

Sri Lanka

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