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Sequence Independent Self-Assembly of Germ Granule mRNAs into Homotypic Clusters

78 Pages Posted: 6 Dec 2019 Sneak Peek Status: Review Complete

See all articles by Tatjana Trcek

Tatjana Trcek

Johns Hopkins University - Department of Biology

Tyler E. Douglas

New York University (NYU) - Department of Cell Biology

Markus Grosch

Helmholtz Association of German Research Centres - Institute of Stem Cell Research (ISF)

Yandong Yin

New York University (NYU) - Department of Biochemistry and Pharmacology

Whitby V.I. Eagle

Princeton University - Department of Molecular Biology

Elizabeth R. Gavis

Princeton University - Department of Molecular Biology

Hari Shroff

National Institute of Biomedical Imaging and Bioengineering - Section on High Resolution Optical Imaging

Eli Rothenberg

New York University (NYU) - Department of Biochemistry and Molecular Pharmacology

Ruth Lehmann

New York University (NYU) - Department of Cell Biology

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Abstract

mRNAs enriched in membraneless condensates provide functional compartmentalization within cells. How mRNAs sort and organize within these condensates is poorly understood. Here, we report on a self-sorting mechanism by which multiple mRNAs derived from the same gene assemble into homotypic mRNA clusters. In Drosophila germ granules, clusters formed by mRNAs originating from different genes are de-mixed from each other within the same granule and located at distinct positions within the homogeneously-distributed protein environment of the granule. The specificity for self-sorting is not dependent on any particular RNA sequence and the intermolecular RNA:RNA interactions among clustered mRNAs are transient. We propose that the ability of mRNAs to self-sort into homotypic assemblies is their inherent property that is augmented under conditions that increase local mRNA concentration, such as upon enrichment in RNA-protein condensates, a process that appears conserved in diverse cellular contexts and organisms.

Keywords: RNA self-assembly, RNA clusters, RNA:RNA interactions, RNA granules, germ granules, phase separation, nos, osk

Suggested Citation

Trcek, Tatjana and Douglas, Tyler E. and Grosch, Markus and Yin, Yandong and Eagle, Whitby V.I. and Gavis, Elizabeth R. and Shroff, Hari and Rothenberg, Eli and Lehmann, Ruth, Sequence Independent Self-Assembly of Germ Granule mRNAs into Homotypic Clusters (December 3, 2019). Available at SSRN: https://ssrn.com/abstract=3497742 or http://dx.doi.org/10.2139/ssrn.3497742
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Tatjana Trcek

Johns Hopkins University - Department of Biology

Mudd Hall 144
3400 N. Charles Street
Baltimore, MD 21218
United States

Tyler E. Douglas

New York University (NYU) - Department of Cell Biology

United States

Markus Grosch

Helmholtz Association of German Research Centres - Institute of Stem Cell Research (ISF)

Ingolstädter Landstraße 1
Neuherberg, D-85764
Germany

Yandong Yin

New York University (NYU) - Department of Biochemistry and Pharmacology ( email )

United States

Whitby V.I. Eagle

Princeton University - Department of Molecular Biology ( email )

119 Lewis Thomas Laboratory
Washington Road
Princeton, NJ 08544-1014
United States

Elizabeth R. Gavis

Princeton University - Department of Molecular Biology ( email )

119 Lewis Thomas Laboratory
Washington Road
Princeton, NJ 08544-1014
United States

Hari Shroff

National Institute of Biomedical Imaging and Bioengineering - Section on High Resolution Optical Imaging ( email )

United States

Eli Rothenberg

New York University (NYU) - Department of Biochemistry and Molecular Pharmacology ( email )

United States

Ruth Lehmann (Contact Author)

New York University (NYU) - Department of Cell Biology ( email )

United States

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