Decellularized Porcine Cornea-Based Hydrogels for Regeneration of Corneal Epithelium and Stroma

48 Pages Posted: 15 Jan 2020

See all articles by Fuyan Wang

Fuyan Wang

Shandong University - Department of Ophthalmology

Weiyun Shi

Shandong First Medical University - State Key Laboratory Cultivation Base

Hua Li

Shandong University - Department of Ophthalmology

Long Zhao

Shandong First Medical University - State Key Laboratory Cultivation Base

Dapeng Sun

Shandong First Medical University - State Key Laboratory Cultivation Base

Hongwei Wang

Shandong First Medical University - State Key Laboratory Cultivation Base

Lingling Yang

Shandong First Medical University - State Key Laboratory Cultivation Base

Ting Liu

Shandong First Medical University - State Key Laboratory Cultivation Base

Qingjun Zhou

Shandong First Medical University - State Key Laboratory Cultivation Base

Lixin Xie

Shandong First Medical University - State Key Laboratory Cultivation Base

Abstract

Hydrogels derived from decellularized tissues provide superior biocompatibility, tenability and tissue-specific extracellular matrix (ECM) components. Based on the preparation of decellularized porcine cornea (DPC), here we developed an injectable hydrogel, characterized the biocompatibility and transparency, and evaluated the use to repair corneal stromal defect in rabbit. Upon cross-linking with N-cyclohexyl-N′-(2-morpholinethyl) carbodiimide metho-p-toluenesulfonate (CMC), the DPC-based hydrogel assumed favorable transparency, exhibited distinct mechanical properties and preserved the ECM components of native porcine cornea (NPC). In vitro experiments showed that the hydrogel maintained the phenotype, supported the proliferation and promoted the ECM synthesis of corneal fibroblasts. When injected on rabbit corneas, the hydrogel rapidly covered, solidified and formed a smooth surface upon the focal defect. Corneal epithelium was fully regenerated within 3 days. The thickness of corneal epithelium and stroma was restored at 12 weeks postoperatively without significant inflammation and scar formation. Notably, the hydrogel showed no harmful effects on the resident stroma and endothelium. Considering to the advantages of low cost and ease of production and use, the DPC-based hydrogel may represent a promising biomaterial for corneal epithelial and stromal regeneration.

Keywords: Decellularized porcine cornea, hydrogel, corneal regeneration, extracellular matrix

Suggested Citation

Wang, Fuyan and Shi, Weiyun and Li, Hua and Zhao, Long and Sun, Dapeng and Wang, Hongwei and Yang, Lingling and Liu, Ting and Zhou, Qingjun and Xie, Lixin, Decellularized Porcine Cornea-Based Hydrogels for Regeneration of Corneal Epithelium and Stroma. Available at SSRN: https://ssrn.com/abstract=3518167 or http://dx.doi.org/10.2139/ssrn.3518167

Fuyan Wang

Shandong University - Department of Ophthalmology

China

Weiyun Shi

Shandong First Medical University - State Key Laboratory Cultivation Base ( email )

China

Hua Li

Shandong University - Department of Ophthalmology

China

Long Zhao

Shandong First Medical University - State Key Laboratory Cultivation Base

China

Dapeng Sun

Shandong First Medical University - State Key Laboratory Cultivation Base

China

Hongwei Wang

Shandong First Medical University - State Key Laboratory Cultivation Base

China

Lingling Yang

Shandong First Medical University - State Key Laboratory Cultivation Base ( email )

China

Ting Liu

Shandong First Medical University - State Key Laboratory Cultivation Base

China

Qingjun Zhou (Contact Author)

Shandong First Medical University - State Key Laboratory Cultivation Base ( email )

China

Lixin Xie

Shandong First Medical University - State Key Laboratory Cultivation Base ( email )

China

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