Anti-Glycation and Antioxidant Potential of Clerodendrum glandulosum Lindl. in the Leaf Extract and Its Fractions
Posted: 3 Feb 2020 Last revised: 8 May 2020
Date Written: January 30, 2020
Abstract
Advanced glycated end products (AGEs) are generated by a non-enzymatic reaction between the amino groups of proteins, lipids, and nucleic acids with reducing sugars. AGEs cause oxidative stress, which can lead to life-threatening conditions and irreversible changes in our body. Inhibition of AGEs and oxidative stress are considered to be of therapeutic potential to prevent such complications. Clerodendrum glandulosum (CG) (syn. Clerodendrum colebrookianum) locally known as Nephaphu in the Assamese is a plant with immense medicinal properties among the tribal communities of North East India. CG leaves were extracted using Methanol-Water (8:2) (MWEx), and this was further fractionated into four fractions, of which two fractions viz., Ethyl Acetate Fraction (EAF) and n-Butanol Fraction (NBF) were found to possess active anti-glycation and antioxidant properties. To evaluate the anti-glycation activity of CG leaf, BSA-fructose anti-glycation assay (Wang et al., 2011) was performed. It was found that at the concentrations of 500µg, MWEx, EAF, and NBF exhibited potent in vitro anti-glycation properties with a percentage inhibition of 75.366 ± 0.397%, 71.498 ± 1.897% and 81.859 ± 1.638% respectively. 2, 2-diphenyl-1-picryl-hydrazyl-hydrate radical scavenging (DPPH), Ferric Reducing Antioxidant Power (FRAP), and phosphomolybdenum assays were performed for measuring the antioxidant activities. The MWEx, EAF and NBF exhibited significant antioxidant activities with IC50s of 51.608 ± 1.732, 36.651 ± 1.089, 68.509 ± 2.253µg/ml for DPPH radical scavenging assay, 57.938 ± 0.291, 41.855 ± 2.075, 48.809 ± 0.567µg/ml for FRAP assay and 52.284 ± 1.521, 37.227 ± 0.143, 45.974 ± 0.478µg/ml for phosphomolybdenum assay respectively. As the extract and its fractions exhibit significant antioxidant activities and inhibit the glycation reaction, it can prevent complications related to AGEs.
Keywords: Anti-glycation, Antioxidants, Advanced-glycated end product (AGE), Clerodendrum glandulosum
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