Inhibiting the Haemolytic Activity of Leptospira Interrogans Using Various Plant-Derived Compounds of Eclipta Alba Via Computational Approach.

Abstract

Leptospirosis is a globally spread zoonotic infectious diseases that bacteria caused by the genus Leptospira, which is a gram-negative spirochete. This Leptospirosis are infected to humans through fresh wounds. Uniquness of this Leptospira; capability to escape from the immunogenic response of the host by hiding themselves in proximal tubules of the kidney and liver for there survivability. Even though Leptospirosis capable of utilizing glucose they feed fatty acids as the there only source of carbon and energy. As Leptospira feed fatty acid on the surface of red blood cells causes parasitic hemolysis leads to multiple organ failure and also by explosive of this cell lysis leads to a mechanism for the biogenesis of membrane vesicles and biofilm formation. The major fractions of the proteome of Leptospira are hypothetical proteins, constrain their identification and characterization to disclose their role in the pathogenesis. In Present studies in-silico characterization approach analyzing the hemolytic proteins of Leptospira interrogans and treating with Eclipta Alba plant for Anti – hemolytic activity were subjected to docking studies to know the binding interactions between them using bioinformatics tools. The protein stability, fluctuations and protein ligand interactions were investigated through molecular dynamics stimulation studies and there potential lead molecular was studied.

Keywords: Leptospirosis, Anti-hemolytic, 2, Eclipta Alba plant, In-Silico Characterization, Hypothetical proteins