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Rapid Detection of Novel Coronavirus (COVID19) by Reverse Transcription-Loop-Mediated Isothermal Amplification

19 Pages Posted: 19 Feb 2020

See all articles by Laura E. Lamb

Laura E. Lamb

Beaumont Health System - Department of Urology; Oakland University - William Beaumont School of Medicine

Sarah N. Bartolone

Beaumont Health System - Department of Urology

Elijah Ward

Beaumont Health System - Department of Urology

Michael B. Chancellor

Beaumont Health System - Department of Urology

More...

Abstract

Novel Corona virus (COVID-19 or 2019-nCoV) is an emerging global health concern that requires a rapid diagnostic test. Quantitative reverse transcription PCR (qRT-PCR) is currently the standard for COVID-19 detection; however, Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) may allow for faster and cheaper field based testing at point-of-risk. The objective of this study was to develop a rapid screening diagnostic test that could be completed in under 30 minutes. Simulated patient samples were generated by spiking serum, urine, saliva, oropharyngeal swabs, and nasopharyngeal swabs with a portion of the COVID-19 nucleic sequence. The samples were tested using RT-LAMP as well as by conventional qRT-PCR. Specificity of the RT-LAMP was evaluated by also testing against other related coronaviruses. RT-LAMP specifically detected COVID-19 in simulated patient samples. This test was performed in under 30 minutes. This approach could be used for monitoring of exposed individuals or potentially aid with screening efforts in the field and potential ports of entry.

Funding Statement: This work was supported by the Maureen and Ronald Hirsch family philanthropic contribution.

Declaration of Interests: The authors declare no competing interests exist.

Ethics Approval Statement: All studies were approved by Beaumont Health’s Institutional Review Board (IRB approval # 2020-040 and 2016-044). All study participants gave their informed consent to participate.

Keywords: Point-of-Care Testing; novel coronavirus; COVID-19; rapid testing

Suggested Citation

Lamb, Laura E. and Bartolone, Sarah N. and Ward, Elijah and Chancellor, Michael B., Rapid Detection of Novel Coronavirus (COVID19) by Reverse Transcription-Loop-Mediated Isothermal Amplification (2/14/2020). Available at SSRN: https://ssrn.com/abstract=3539654 or http://dx.doi.org/10.2139/ssrn.3539654

Laura E. Lamb

Beaumont Health System - Department of Urology ( email )

Royal Oak, MI
United States

Oakland University - William Beaumont School of Medicine ( email )

Rochester Hills, MI
United States

Sarah N. Bartolone

Beaumont Health System - Department of Urology

Royal Oak, MI
United States

Elijah Ward

Beaumont Health System - Department of Urology

Royal Oak, MI
United States

Michael B. Chancellor (Contact Author)

Beaumont Health System - Department of Urology

Royal Oak, MI
United States

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