lancet-header
Preprints with The Lancet is part of SSRN´s First Look, a place where journals and other research experts identify content of interest prior to publication. These preprint papers are not peer-reviewed and are posted here as part of a 12-month trial. Authors have either opted in at submission to The Lancet family of journals to post their preprints on Preprints with The Lancet, or submitted directly via SSRN. The usual SSRN checks and a Lancet-specific check for appropriateness and transparency have been applied. These papers should not be used for clinical decision making or reporting of research to a lay audience without indicating that this is preliminary research that has not been peer-reviewed. For more information see the Comment published in The Lancet, or visit The Lancet´s FAQ page, and for any feedback please contact preprints@lancet.com

Characteristics of Peripheral Lymphocyte Subset Alteration in 2019-nCoV Pneumonia

21 Pages Posted: 24 Feb 2020

See all articles by Fan Wang

Fan Wang

Wuhan University - Department of Gastroenterology

Jiayan Nie

Wuhan University - Department of Gastroenterology

Hongling Wang

Wuhan University - Department of Gastroenterology

Yu Xiao

Wuhan University - Department of Biological Repositories

Haizhou Wang

Wuhan University - Department of Gastroenterology

Xiaoping Liu

Wuhan University - Department of Urology

Liping Deng

Wuhan University - Department of Infectious Disease

Yuanyuan Xing

Wuhan University - Department of Biological Repositories

Tielong Chen

Wuhan University - Department of Infectious Disease

Xiaoping Chen

Wuhan University - Department of Infectious Disease

Shihui Song

Wuhan University - Department of Infectious Disease

Zhenshun Cheng

Wuhan University - Department of Respiratory Medicine

Junli Fan

Wuhan University - Department of Laboratory Medicine

Hengning Ke

Wuhan University - Department of Infectious Disease

Shicheng Gao

Wuhan University - Department of Infectious Disease

Jin Li

Wuhan University - Department of Gastroenterology

Min Chen

Wuhan University - Department of Gastroenterology

Liping Chen

Wuhan University - Department of Gastroenterology

Ying Chang

Wuhan University - Department of Gastroenterology

Rui Zhou

Wuhan University - Department of Gastroenterology

Jun Fang

Wuhan University - Department of Gastroenterology

Jing Liu

Wuhan University - Department of Gastroenterology

Xiaobing Wang

Wuhan University - Department of Gastroenterology

Fei Xu

Wuhan University - Department of Gastroenterology

Xing Huang

Wuhan University - Department of Digestive Endoscopy

Jing Liu

Wuhan University - Emergency Center

Pengfei Chen

Central Hospital of Enshi Autonomous Prefecture - Department of Gastroenterology

Guozhen Li

Wuhan Red Cross Hospital - Department of Gastroenterology

Jun Lin

Wuhan University - Department of Gastroenterology

Xinghuan Wang

Wuhan University - Department of Urology

Qiu Zhao

Wuhan University - Department of Gastroenterology

Yong Xiong

Wuhan University - Department of Infectious Disease

Pingzheng Mo

Wuhan University - Department of Infectious Disease

Yongxi Zhang

Wuhan University - Department of Infectious Disease

More...

Abstract

Background: Since December 2019, novel coronavirus (2019-nCoV)-infected pneumonia (NCP) occurred in Wuhan, and rapidly spread throughout China. We aimed to clarify the characteristics and clinical significance of peripheral lymphocyte subset alteration in NCP.

Methods: The levels of peripheral lymphocyte subsets were measured by flow cytometry in 60 NCP patients before and after treatment, and their association with clinical characteristics and efficacy was analyzed.

Findings: Compared to healthy controls, NCP patients had a significant decrease of total lymphocytes, CD4+ T cells, CD8+ T cells, B cells and natural killer (NK) cells. In NCP patients, severe cases had a lower level of CD4+ T cells and CD8+ T cells than mild cases. CD8+ T cell levels were negatively correlated with inflammatory indicators of erythrocyte sedimentation rat (ESR), C-reactive protein (CRP) and interleukin-6 (IL-6), while CD4+/CD8+ ratio was positively correlated. Levels of total lymphocytes and CD4+ T cells were negatively correlated with ESR, and NK cells in negative correlation with IL-6. After one-week treatment, 37 patients (67%) reached clinical response, and had a significant increase of total lymphocytes, CD8+ T cells and B cells. In multivariate analysis, post-treatment decrease of CD8+ T cells and B cells and increase of CD4+/CD8+ ratio were indicated as independent predictors for poor clinical efficacy.

Interpretation: Peripheral lymphocyte subset alteration demonstrated a significant association with the clinical characteristics and efficacy of NCP. CD8+ T cells tended to be an independent predictor for NCP severity and treatment efficacy.

Funding Statement: This study was supported by the Program of Excellent Doctoral (Postdoctoral) of Zhongnan Hospital of Wuhan University (Grant No. ZNYB2019003).

Declaration of Interests: The authors declared no competing interests.

Ethics Approval Statement: This study was approved by the ethics committee of Zhongnan Hospital of Wuhan University (No. 2020011). Written informed consent was obtained from patients.

Keywords: 2019 novel coronavirus; pneumonia; lymphocyte subset

Suggested Citation

Wang, Fan and Nie, Jiayan and Wang, Hongling and Xiao, Yu and Wang, Haizhou and Liu, Xiaoping and Deng, Liping and Xing, Yuanyuan and Chen, Tielong and Chen, Xiaoping and Song, Shihui and Cheng, Zhenshun and Fan, Junli and Ke, Hengning and Gao, Shicheng and Li, Jin and Chen, Min and Chen, Liping and Chang, Ying and Zhou, Rui and Fang, Jun and Liu, Jing and Wang, Xiaobing and Xu, Fei and Huang, Xing and Liu, Jing and Chen, Pengfei and Li, Guozhen and Lin, Jun and Wang, Xinghuan and Zhao, Qiu and Xiong, Yong and Mo, Pingzheng and Zhang, Yongxi, Characteristics of Peripheral Lymphocyte Subset Alteration in 2019-nCoV Pneumonia (2/16/2020). Available at SSRN: https://ssrn.com/abstract=3539681 or http://dx.doi.org/10.2139/ssrn.3539681

Fan Wang

Wuhan University - Department of Gastroenterology

Wuhan, 430071
China

Jiayan Nie

Wuhan University - Department of Gastroenterology

Wuhan, 430071
China

Hongling Wang

Wuhan University - Department of Gastroenterology

Wuhan, 430071
China

Yu Xiao

Wuhan University - Department of Biological Repositories

Wuhan
China

Haizhou Wang

Wuhan University - Department of Gastroenterology

Wuhan, 430071
China

Xiaoping Liu

Wuhan University - Department of Urology

China

Liping Deng

Wuhan University - Department of Infectious Disease

China

Yuanyuan Xing

Wuhan University - Department of Biological Repositories

Wuhan
China

Tielong Chen

Wuhan University - Department of Infectious Disease

China

Xiaoping Chen

Wuhan University - Department of Infectious Disease

China

Shihui Song

Wuhan University - Department of Infectious Disease

China

Zhenshun Cheng

Wuhan University - Department of Respiratory Medicine

Wuhan, 430071
China

Junli Fan

Wuhan University - Department of Laboratory Medicine

Wuhan, 430071
China

Hengning Ke

Wuhan University - Department of Infectious Disease

China

Shicheng Gao

Wuhan University - Department of Infectious Disease

China

Jin Li

Wuhan University - Department of Gastroenterology

Wuhan, 430071
China

Min Chen

Wuhan University - Department of Gastroenterology

Wuhan, 430071
China

Liping Chen

Wuhan University - Department of Gastroenterology

Wuhan, 430071
China

Ying Chang

Wuhan University - Department of Gastroenterology

Wuhan, 430071
China

Rui Zhou

Wuhan University - Department of Gastroenterology

Wuhan, 430071
China

Jun Fang

Wuhan University - Department of Gastroenterology

Wuhan, 430071
China

Jing Liu

Wuhan University - Department of Gastroenterology

Wuhan, 430071
China

Xiaobing Wang

Wuhan University - Department of Gastroenterology

Wuhan, 430071
China

Fei Xu

Wuhan University - Department of Gastroenterology

Wuhan, 430071
China

Xing Huang

Wuhan University - Department of Digestive Endoscopy

Wuhan, 430071
China

Jing Liu

Wuhan University - Emergency Center

China

Pengfei Chen

Central Hospital of Enshi Autonomous Prefecture - Department of Gastroenterology

Enshi, 445000
China

Guozhen Li

Wuhan Red Cross Hospital - Department of Gastroenterology

Wuhan, 430015
China

Jun Lin

Wuhan University - Department of Gastroenterology

Wuhan, 430071
China

Xinghuan Wang

Wuhan University - Department of Urology

China

Qiu Zhao

Wuhan University - Department of Gastroenterology ( email )

Wuhan, 430071
China

Yong Xiong

Wuhan University - Department of Infectious Disease ( email )

China

Pingzheng Mo

Wuhan University - Department of Infectious Disease ( email )

China

Yongxi Zhang (Contact Author)

Wuhan University - Department of Infectious Disease ( email )

China

Click here to go to TheLancet.com

Paper statistics

Abstract Views
621
Downloads
112