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Longitudinal Characteristics of Lymphocyte Responses and Cytokine Profiles in the Peripheral Blood of SARS-CoV-2 Infected Patients

37 Pages Posted: 19 Feb 2020

See all articles by Jing Liu

Jing Liu

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Sumeng Li

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Jia Liu

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Boyun Liang

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Xiaobei Wang

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Clinical Laboratory

Wei Li

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Hua Wang

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Qiaoxia Tong

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Jianhua Yi

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Lei Zhao

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Lijuan Xiong

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Chunxia Guo

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Jin Tian

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Jinzhuo Luo

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Jinghong Yao

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Ran Pang

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Hui Shen

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Cheng Peng

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Ting Liu

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Qian Zhang

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Jun Wu

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Ling Xu

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Sihong Lu

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Baoju Wang

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Zhihong Weng

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Chunrong Han

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Huabing Zhu

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Ruxia Zhou

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Helong Zhou

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Xiliu Chen

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Pian Ye

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Bin Zhu

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Shengsong He

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Yongwen He

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Shenghua Jie

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Ping Wei

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Jianao Zhang

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Yinping Lu

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Weixian Wang

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Li Zhang

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Ling Li

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Fengqin Zhou

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Jun Wang

University of Duisburg-Essen - Institute for Virology

Ulf Dittmer

University of Duisburg-Essen - Institute for Virology

Mengji Lu

University of Duisburg-Essen - Institute for Virology

Yu Hu

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Institute of Hematology; Huazhong University of Science and Technology (Formerly Tongi Medical University) - Collaborative Innovation Center of Hematology

Dongliang Yang

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Xin Zheng

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

More...

Abstract

Background: The dynamic changes of lymphocyte subsets and cytokines profiles of patients with novel coronavirus disease (COVID-19) and their correlation with the disease severity remain unclear.  

Method: Peripheral blood samples were longitudinally collected from 40 confirmed COVID-19 patients and examined for lymphocyte subsets by flow cytometry and cytokine profiles by specific immunoassays.  

Findings: Of the 40 COVID-19 patients enrolled, 13 severe cases showed significant and sustained decreases in lymphocyte counts but increases in neutrophil counts than 27 mild cases. Further analysis demonstrated significant decreases in the counts of T cells, especially CD8 + T cells, as well as increases in IL-6, IL-10, IL-2 and IFN-γ levels in the peripheral blood in the severe cases compared to those in the mild cases. T cell counts and cytokine levels in severe COVID-19 patients who survived the disease gradually recovered at later time points to levels that were comparable to those of the mild cases. Moreover, the neutrophil-to-CD8+ T cell ratio (N8R) were identified as the most powerful prognostic factor affecting the prognosis for severe COVID-19.  

Interpretation: The degree of lymphopenia and a proinflammatory cytokine storm is higher in severe COVID-19 patients than in mild cases, and is associated with the disease severity. N8R may serve as a useful prognostic factor for early identification of severe COVID-19 cases.  

Funding Statement: This work is supported by the National Natural Science Foundation of China (81861138044, 91742114 and 91642118), the National Science and Technology Major Project (2018ZX10723203, 2018ZX10302206, 2017ZX10202201, 2017ZX10202202 and 2017ZX10202203), the Innovation Team Project of Health Commission of Hubei Province (WJ2019C003), the Integrated Innovative Team for Major Human Diseases Program of Tongji Medical College and the “Double-First Class” Project for the International Cooperation Center on Infection and Immunity, HUST, and a special joint project of University Hospital Essen, University of Duisburg-Essen.

Declaration of Interests: The authors disclose no conflicts of interest.

Ethics Approval Statement: A written informed consent was regularly obtained from all patients upon admission into Wuhan Union Hospital, China. The study was approved by the Ethics Committee of Tongji Medical College of Huazhong University of Science and Technology.

Keywords: lymphocyte responses, cytokine profiles, SARS-CoV-2

Suggested Citation

Liu, Jing and Li, Sumeng and Liu, Jia and Liang, Boyun and Wang, Xiaobei and Li, Wei and Wang, Hua and Tong, Qiaoxia and Yi, Jianhua and Zhao, Lei and Xiong, Lijuan and Guo, Chunxia and Tian, Jin and Luo, Jinzhuo and Yao, Jinghong and Pang, Ran and Shen, Hui and Peng, Cheng and Liu, Ting and Zhang, Qian and Wu, Jun and Xu, Ling and Lu, Sihong and Wang, Baoju and Weng, Zhihong and Han, Chunrong and Zhu, Huabing and Zhou, Ruxia and Zhou, Helong and Chen, Xiliu and Ye, Pian and Zhu, Bin and He, Shengsong and He, Yongwen and Jie, Shenghua and Wei, Ping and Zhang, Jianao and Lu, Yinping and Wang, Weixian and Zhang, Li and Li, Ling and Zhou, Fengqin and Wang, Jun and Dittmer, Ulf and Lu, Mengji and Hu, Yu and Yang, Dongliang and Zheng, Xin, Longitudinal Characteristics of Lymphocyte Responses and Cytokine Profiles in the Peripheral Blood of SARS-CoV-2 Infected Patients (2/16/2020). Available at SSRN: https://ssrn.com/abstract=3539682 or http://dx.doi.org/10.2139/ssrn.3539682

Jing Liu

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Sumeng Li

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Jia Liu

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Boyun Liang

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Xiaobei Wang

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Clinical Laboratory

China

Wei Li

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Hua Wang

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Qiaoxia Tong

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Jianhua Yi

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Lei Zhao

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases ( email )

Wuhan
China

Lijuan Xiong

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Chunxia Guo

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Jin Tian

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Jinzhuo Luo

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Jinghong Yao

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Ran Pang

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Hui Shen

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Cheng Peng

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Ting Liu

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Qian Zhang

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Jun Wu

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Ling Xu

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Sihong Lu

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Baoju Wang

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Zhihong Weng

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Chunrong Han

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Huabing Zhu

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Ruxia Zhou

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Helong Zhou

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Xiliu Chen

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Pian Ye

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Bin Zhu

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Shengsong He

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Yongwen He

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Shenghua Jie

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Ping Wei

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Jianao Zhang

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Yinping Lu

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Weixian Wang

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Li Zhang

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Ling Li

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Fengqin Zhou

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases

Wuhan
China

Jun Wang

University of Duisburg-Essen - Institute for Virology

Hufelandstraße 55
45147 Essen
Germany

Ulf Dittmer

University of Duisburg-Essen - Institute for Virology ( email )

Hufelandstraße 55
45147 Essen
Germany

Mengji Lu

University of Duisburg-Essen - Institute for Virology

Hufelandstraße 55
45147 Essen
Germany

Yu Hu

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Institute of Hematology ( email )

Wuhan
China

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Collaborative Innovation Center of Hematology ( email )

Wuhan
China

Dongliang Yang

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases ( email )

Wuhan
China

Xin Zheng (Contact Author)

Huazhong University of Science and Technology (Formerly Tongi Medical University) - Department of Infectious Diseases ( email )

Wuhan
China

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