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Conjunctival Polymerase Chain Reaction-Tests of 2019 Novel Coronavirus in Patients in Shenyang, China

22 Pages Posted: 28 Feb 2020

See all articles by Li Xu

Li Xu

The Fourth People’s Hospital of Shenyang

Xinyue Zhang

China Medical University - Shengjing Hospital

Wei Song

Collaborative Innovation Center for Diagnosis Treatment of Infectious Diseases - Chinese Center for Disease Control and Prevention (China CDC)

Baijun Sun

Collaborative Innovation Center for Diagnosis Treatment of Infectious Diseases - Chinese Center for Disease Control and Prevention (China CDC)

Jinping Mu

Collaborative Innovation Center for Diagnosis Treatment of Infectious Diseases - Chinese Center for Disease Control and Prevention (China CDC)

Bing Wang

Collaborative Innovation Center for Diagnosis Treatment of Infectious Diseases - Chinese Center for Disease Control and Prevention (China CDC)

Zhiqian Wang

The Fourth People’s Hospital of Shenyang

Yehong Cao

The Fourth People’s Hospital of Shenyang

Xue Dong

Collaborative Innovation Center for Diagnosis Treatment of Infectious Diseases - Chinese Center for Disease Control and Prevention (China CDC)

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Abstract

Background: The 2019 novel coronavirus(COVID-19) mainly transmitted by person-to-person through inhalation of respiratory droplets. We report the laboratory results of conjunctival PCR-tests and some clinical features of these patients in Shenyang China.

Methods: All patients with diagnosed and suspected COVID-19 were admitted to a designated hospital in Shenyang,China. We collected conjunctival samples of these patients to do the laboratory tests by real time RT-PCR.Medical observed patients were enrolled if they had clinical symptoms. Then we analysed the PCR results and clinical data from eletronic medical records in order to find some relationships.

Findings: The study include 14 confirmly diagnosed cases,16 suspected cases and some medical observed patients.One of the identified case coverted from suspected case without typical clinical symptoms.Twenty-two medical observed cases were removed because none of them converted to identified cases.One of the suspected converted to identified case recently.The included cases in our study are imported cases with less underlying diseases and the severity of their infection was relatively moderate. All the conjunctival results of PCR-test were negative.Two cases had typical clinical symptoms but were finally confirmed by repeated pharynx swab tests.

Interpretation: Conjunctiva may be a transmission way of COVID-19.And ocular conjunctival swabs in combination with PCR test could be a non-invasive,convenient and feasible diagnostic method for identifying the infection of COVID-19.Emphasis on the false-negative results is vital.

Funding Statement: Ministry of Shenyang Science and Technology.

Declaration of Interests: The authors reported that no conflicting relationship exists for any author.

Ethics Approval Statement: The study was approved by local ethics committee, sponsored by Ministry of Shenyang Science and Technology and in accordance with Declaration of Helsinki.

Keywords: COVID-19; PCR; Conjunctival swab

Suggested Citation

Xu, Li and Zhang, Xinyue and Song, Wei and Sun, Baijun and Mu, Jinping and Wang, Bing and Wang, Zhiqian and Cao, Yehong and Dong, Xue, Conjunctival Polymerase Chain Reaction-Tests of 2019 Novel Coronavirus in Patients in Shenyang, China (2/20/2020). Available at SSRN: https://ssrn.com/abstract=3543592 or http://dx.doi.org/10.2139/ssrn.3543592

Li Xu (Contact Author)

The Fourth People’s Hospital of Shenyang ( email )

Shenyang
China

Xinyue Zhang

China Medical University - Shengjing Hospital

Shenyang, Liaoning 110004
China

Wei Song

Collaborative Innovation Center for Diagnosis Treatment of Infectious Diseases - Chinese Center for Disease Control and Prevention (China CDC)

155 Changbai Road
Changping District
Beijing, Changping District 102206
China

Baijun Sun

Collaborative Innovation Center for Diagnosis Treatment of Infectious Diseases - Chinese Center for Disease Control and Prevention (China CDC)

155 Changbai Road
Changping District
Beijing, Changping District 102206
China

Jinping Mu

Collaborative Innovation Center for Diagnosis Treatment of Infectious Diseases - Chinese Center for Disease Control and Prevention (China CDC)

155 Changbai Road
Changping District
Beijing, Changping District 102206
China

Bing Wang

Collaborative Innovation Center for Diagnosis Treatment of Infectious Diseases - Chinese Center for Disease Control and Prevention (China CDC)

155 Changbai Road
Changping District
Beijing, Changping District 102206
China

Zhiqian Wang

The Fourth People’s Hospital of Shenyang

Shenyang
China

Yehong Cao

The Fourth People’s Hospital of Shenyang

Shenyang
China

Xue Dong

Collaborative Innovation Center for Diagnosis Treatment of Infectious Diseases - Chinese Center for Disease Control and Prevention (China CDC)

155 Changbai Road
Changping District
Beijing, Changping District 102206
China

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