lancet-header
Preprints with The Lancet is part of SSRN´s First Look, a place where journals and other research experts identify content of interest prior to publication. These preprint papers are not peer-reviewed and are posted here as part of a 12-month trial. Authors have either opted in at submission to The Lancet family of journals to post their preprints on Preprints with The Lancet, or submitted directly via SSRN. The usual SSRN checks and a Lancet-specific check for appropriateness and transparency have been applied. These papers should not be used for clinical decision making or reporting of research to a lay audience without indicating that this is preliminary research that has not been peer-reviewed. For more information see the Comment published in The Lancet, or visit The Lancet´s FAQ page, and for any feedback please contact preprints@lancet.com

Immunoglobulin G/M and Cytokines Detections in Continuous Sera from Patients with Novel Coronaviruses (2019-nCoV) Infection

23 Pages Posted: 28 Feb 2020

See all articles by Bo Li

Bo Li

Government of the People's Republic of China - Department of Clinical Laboratory

Fan Feng

Government of the People's Republic of China - Department of Clinical Laboratory

Guang Yang

Government of the People's Republic of China - Department of Clinical Laboratory

Aixia Liu

Government of the People's Republic of China - Department of Clinical Laboratory

Ning Yang

Government of the People's Republic of China - Department of Clinical Laboratory

Qiyu Jiang

Government of the People's Republic of China - Department of Clinical Laboratory

Hao Zhang

Government of the People's Republic of China - Department of Clinical Laboratory

Tao Wang

Government of the People's Republic of China - Department of Clinical Laboratory

Peiran Li

Government of the People's Republic of China - Department of Clinical Laboratory

Yuanli Mao

Government of the People's Republic of China - Department of Clinical Laboratory

Boan Li

Government of the People's Republic of China - Department of Clinical Laboratory

More...

Abstract

Background: An outbreak of severe respiratory illness caused by a novel coronaviruses (2019- nCoV) in China since December 2019. This work aim to evaluate the clinical diagnostic values of the Immunoglobulin G/M’s or the inflammatory-related factor’s level in patients’ serum sample and the relationship with clinical manifestations and epidemiologic features.

Methods: A total of forty-nine patients confirmed by the nucleic acid test were enrolled in this study, the S (Spike) and N (Nucleocapsid) -based G/M neutralizing antibodies were detected in 206 continuous serum samples by using the enzyme-linked immunosorbent assay (ELISA). The inflammatory-ratelted factors, including interleukin-2 (IL-2), interleukin-4 (IL-4), interleukin6 (IL-6), interleukin-10 (IL-10), interleukin-17 (IL-17), interleukin-12p70 (IL-12p70), tumor necrosis factor-α(TNF-α) and interferon-γ(INF-γ) were determined by a flow cytometry.

Results: As regards to S-IgM, N-IgM, S-IgG and N-IgG, the detection positive rates in 49 patients were 38.8%, 51.0%,63.3% and 40.8% respectively. The median detection times for those antibodies were the 10th, 9th, 9th, or 9th days’ time point after the illness onset. The Kappa test results showed that the consistency of two IgM detection and two IgG detection are poor, the Kappa index are 0.408 and 0.344 respectively. 73.5%, 14.3% and 10.2% patients had high levels IL-6, IL-10 and INF-γ results. We also found that patients who lived in Wuhan or traveling to Wuhan in the short term had higher S-IgM (p=0.045) and N-IgM (p=0.002) concentrations than those who stayed in other cities. IL-6 concentrations had significant differences between severe patients and mild patients (p=0.0391). IL-10 concentration elevated in patients with background diseases compared with those no background diseases patients (p=0.0122).

Conclusions: N-IgM and S-IgG detection are more appropriate for use in the early patients screening than S-IgM and N-IgG. Antibodies detection has higher positive rate after 10 days illness onset. IL-6, IL-10 and INF-γ levels can be regarded as novel biomarkers for the prognostic and therapeutic effect evaluation.

Funding Statement: This work was supported by the Emergency Project for 2019-nCoV of PLA General Hospital (20EP013).

Declaration of Interests: The authors stated that there is no conflict of interest.

Ethics Approval Statement: The study procedures were approved by the ethics committee of The Fifth Medical Centre of PLA General Hospital of China.

Keywords: Novel coronaviruses (2019-nCoV); Immunoglobulin G/M detection; cytokines; cytokine storm

Suggested Citation

Li, Bo and Feng, Fan and Yang, Guang and Liu, Aixia and Yang, Ning and Jiang, Qiyu and Zhang, Hao and Wang, Tao and Li, Peiran and Mao, Yuanli and Li, Boan, Immunoglobulin G/M and Cytokines Detections in Continuous Sera from Patients with Novel Coronaviruses (2019-nCoV) Infection (February 21, 2020). Available at SSRN: https://ssrn.com/abstract=3543609 or http://dx.doi.org/10.2139/ssrn.3543609

Bo Li

Government of the People's Republic of China - Department of Clinical Laboratory ( email )

China

Fan Feng

Government of the People's Republic of China - Department of Clinical Laboratory ( email )

China

Guang Yang

Government of the People's Republic of China - Department of Clinical Laboratory

China

Aixia Liu

Government of the People's Republic of China - Department of Clinical Laboratory ( email )

China

Ning Yang

Government of the People's Republic of China - Department of Clinical Laboratory ( email )

China

Qiyu Jiang

Government of the People's Republic of China - Department of Clinical Laboratory ( email )

China

Hao Zhang

Government of the People's Republic of China - Department of Clinical Laboratory

China

Tao Wang

Government of the People's Republic of China - Department of Clinical Laboratory ( email )

China

Peiran Li

Government of the People's Republic of China - Department of Clinical Laboratory ( email )

China

Yuanli Mao

Government of the People's Republic of China - Department of Clinical Laboratory ( email )

China

Boan Li (Contact Author)

Government of the People's Republic of China - Department of Clinical Laboratory ( email )

China

Click here to go to TheLancet.com

Paper statistics

Abstract Views
623
Downloads
186