Neurons are frequently classified into distinct groups or cell types on the basis of structural, physiological, or genetic attributes. To better constrain the definition of neuronal cell types, we characterized the transcriptomes and intrinsic physiological properties of over 3,700 GABAergic mouse visual cortical neurons and reconstructed the local morphologies of 350 of those neurons. We found that most transcriptomic types (t-types) occupy specific laminar positions within mouse visual cortex, and many of those t-types exhibit consistent electrophysiological and morphological features. We observed that these properties could vary continuously between t- types, which limited the ability to predict specific t-types from other data modalities. Despite that, the data support the presence of at least 20 interneuron met-types that have congruent morphological, electrophysiological, and transcriptomic properties.
Gouwens, Nathan W. and Sorensen, Staci A. and Baftizadeh, Fahimeh and Budzillo, Agata and Lee, Brian R. and Jarsky, Tim and Alfiler, Lauren and Baker, Katherine and Barkan, Eliza and Berry, Kyla and Bertagnolli, Darren and Bickley, Kris and Bomben, Jasmine and Braun, Thomas and Brouner, Kirssy and Casper, Tamara and Crichton, Kirsten and Daigle, Tanya L. and Dalley, Rachel and de Frates, Rebeeca and Dee, Nick and Desta, Tsega and Dingman Lee, Samuel and Dotson, Nadezhda and Egdorf, Tom and Ellingwood, Lauren and Enstrom, Rachel and Esposito, Luke and Farrell, Colin and Feng, David and Fong, Olivia and Gala, Rohan and Gamlin, Clare and Gary, Amanda and Glandon, Alexandra and Goldy, Jeff and Gorham, Melissa and Graybuck, Lucas and Gu, Hong and Hadley, Kirsten and Hawrylycz, Michael J. and Henry, Alex M. and Hill, DiJon and Happ, Madie and Kebede, Sara and Kyung Kim, Tae and Kim, Lisa and Kroll, Matthew and Lee, Changkyu and Link, Katherine E. and Mallory, Matthew and Mann, Rusty and Maxwell, Michelle and McGraw, Medea and McMillen, Delissa and Mukora, Alice and Ng, Lindsay and Ng, Lydia and Ngo, Kiet and Nicovich, Philip R. and Oldre, Aaron and Park, Daniel and Peng, Hanchuan and Penn, Osnat and Pham, Thanh and Pom, Alice and Potekhina, Lyida and Rajanbabu, Ramkumar and Ransford, Shea and Reid, David and Rimorin, Christine and Robertson, Miranda and Ronellenfitch, Kara and Ruiz, Augustin and Sandman, David and Smith, Kimberly and Sulc, Josef and Sunkin, Susan M. and Szafer, Aaron and Tieu, Michael and Torkelson, Amy and Trinh, Jessica and Tung, Herman and Wakeman, Wayne and Ward, Katelyn and Williams, Grace and Zhou, Zhi and Ting, Jonathan and Arkhipov, Anton and Sumbul, Uygar and Lein, Ed and Koch, Christof and Yao, Zizhen and Tasic, Bosiljka and Berg, Jim and Murphy, Gabe J. and Zeng, Hongkui, Toward an Integrated Classification of Cell Types: Morphoelectric and Transcriptomic Characterization of Individual GABAergic Cortical Neurons. Available at SSRN: https://ssrn.com/abstract=3544405
This is a paper under consideration at Cell Press and has not been peer-reviewed.