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Single Cell RNA Sequencing Analysis of Human Kidney Reveals the Presence of ACE2 Receptor: A Potential Pathway of COVID-19 Infection

29 Pages Posted: 2 Mar 2020

See all articles by Qiyu He

Qiyu He

Jinan University - First Affiliated Hospital

Tsz Ngai Mok

Jinan University - First Affiliated Hospital

Liang Yun

Southern Medical University - First Medical College

Chengbo He

Heyu Health Technology Co., Ltd.

Jieruo Li

Jinan University - First Affiliated Hospital

Jing Hua Pan

Jinan University - Department of General Surgery

More...

Abstract

Background: A novel coronavirus called COVID-19, which shared 82% similarity of genome sequence with SARS-CoV, was found in Wuhan in late December of 2019, causing an epidemic outbreak of novel coronavirus-induced pneumonia with dramatically increasing number of cases. Several organs are vulnerable to COVID-19 infection. Acute kidney injury (AKI) was reported in parts of case-studies reporting characteristics of COVID-19 patients. This study aimed at analyzing the potential route of COVID-19 entry and mechanism at cellular level.

Methods: cRNA-seq technology was used to obtain evidence of potential route and ACE2 expressing cell in renal system for underlying pathogenesis of kidney injury caused by COVID-19. The whole process was performed under R with Seurat packages. Canonical marker genes were used to annotate different types of cells.

Results: Ten different clusters were identified and ACE2 was mainly expressed in proximal tubule and glomerular parietal epithelial cells. From GO & KEGG enrichment analysis, imbalance of ACE2 expression, renin-angiotensin system (RAS) activation, and neutrophil-related processes were the main issue of COVID-19 leading kidney injury.

Conclusion: Our study provided the cellular evidence that COVID-19 invaded human kidney tissue via proximal convoluted tubule, proximal tubule, proximal straight tubule cells and glomerular parietal cells by means of ACE2-related pathway and used their cellular protease TMPRSS2 for priming.

Funding Statement: The authors stated: "Not available."

Declaration of Interests: All authors have no conflicts of interest to disclose.

Keywords: COVID-19, Virus, scRNA-seq, Kidney, ACE2

Suggested Citation

He, Qiyu and Mok, Tsz Ngai and Yun, Liang and He, Chengbo and Li, Jieruo and Pan, Jing Hua, Single Cell RNA Sequencing Analysis of Human Kidney Reveals the Presence of ACE2 Receptor: A Potential Pathway of COVID-19 Infection (February 24, 2020). Available at SSRN: https://ssrn.com/abstract=3544810 or http://dx.doi.org/10.2139/ssrn.3544810

Qiyu He

Jinan University - First Affiliated Hospital

Guangdong
China

Tsz Ngai Mok

Jinan University - First Affiliated Hospital

Guangdong
China

Liang Yun

Southern Medical University - First Medical College

China

Chengbo He

Heyu Health Technology Co., Ltd.

China

Jieruo Li

Jinan University - First Affiliated Hospital ( email )

Guangdong
China

Jing Hua Pan (Contact Author)

Jinan University - Department of General Surgery ( email )

China

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