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Di-Self-Crosslinking Hsmssa Hydrogel Combined with Colⅰ Constructed Biomimetic Injectable Cartilage Filling Scaffold

36 Pages Posted: 6 Mar 2020 Publication Status: Accepted

See all articles by Ya Yao

Ya Yao

Sichuan University - National Engineering Research Center for Biomaterials

Xing Li

Sichuan University - National Engineering Research Center for Biomaterials

Peilei Wang

Sichuan University - National Engineering Research Center for Biomaterials

Yang Xu

Sichuan University - National Engineering Research Center for Biomaterials

Gonggong Lu

Sichuan University - National Engineering Research Center for Biomaterials

Qing Jiang

Sichuan University - National Engineering Research Center for Biomaterials

Yong Sun

Sichuan University - National Engineering Research Center for Biomaterials

Yujiang Fan

Sichuan University - National Engineering Research Center for Biomaterials

Xingdong Zhang

Sichuan University - National Engineering Research Center for Biomaterials

Abstract

Injectable hydrogels have attracted increasing attention because of convenient clinical operation, non-invasive surgical procedure and seamless filling of irregular defects. Here, injectable di-self-crosslinking HSMSSA hydrogel was formed via fast thiol/maleimide click chemistry reaction and thiol oxidation reaction as primary and secondary self-crosslinking network, respectively. Molecular weight and precursor concentration significantly affected physichemical properties and biological functions of hydrogels. Although this HSMSSA gel (0.1M, 10 mg/mL) had excellent injectability, collagen-like mechanical properties and optimal chondrocytes proliferation efficiency in vitro, and could greatly promote cartilaginous tissue formation in vivo, the lack of adhesion sites resulted in an untenable situation in maintaining effective connections among newborn cell clusters. However, the biomimetic injectable di-self-crosslinking blend hydrogel by combined injectable HSMSSA and bioactive Col I presented excellent biological function in biocompatibility, resistance to degradation, chondrocytes adhesion and proliferation, especially for multiples ascending genes expression level associated with hyaline cartilage formation and polyproteoglycan secretion, which might be an excellent potential clinical treatment strategy for constructing injectable cartilage repair filler by combining expanded autologous chondrocytes.

Keywords: Di-self-crosslinking, Click chemistry, Injectable hydrogel, Cartilage filling scaffold

Suggested Citation

Yao, Ya and Li, Xing and Wang, Peilei and Xu, Yang and Lu, Gonggong and Jiang, Qing and Sun, Yong and Fan, Yujiang and Zhang, Xingdong, Di-Self-Crosslinking Hsmssa Hydrogel Combined with Colⅰ Constructed Biomimetic Injectable Cartilage Filling Scaffold. Available at SSRN: https://ssrn.com/abstract=3548855 or http://dx.doi.org/10.2139/ssrn.3548855

Ya Yao (Contact Author)

Sichuan University - National Engineering Research Center for Biomaterials

Chengdu, Sichuan 610064
China

Xing Li

Sichuan University - National Engineering Research Center for Biomaterials

Chengdu, Sichuan 610064
China

Peilei Wang

Sichuan University - National Engineering Research Center for Biomaterials

Chengdu, Sichuan 610064
China

Yang Xu

Sichuan University - National Engineering Research Center for Biomaterials

Chengdu, Sichuan 610064
China

Gonggong Lu

Sichuan University - National Engineering Research Center for Biomaterials

Chengdu, Sichuan 610064
China

Qing Jiang

Sichuan University - National Engineering Research Center for Biomaterials ( email )

Chengdu, Sichuan 610064
China

Yong Sun

Sichuan University - National Engineering Research Center for Biomaterials ( email )

Chengdu, Sichuan 610064
China

Yujiang Fan

Sichuan University - National Engineering Research Center for Biomaterials

Chengdu, Sichuan 610064
China

Xingdong Zhang

Sichuan University - National Engineering Research Center for Biomaterials

Chengdu, Sichuan 610064
China

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