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Nutrient-Dependent Trade-Offs between Ribosomes and Division Protein Synthesis Control Bacterial Growth and Cell Shape

23 Pages Posted: 12 Mar 2020 Sneak Peek Status: Under Review

See all articles by Diana Serbanescu

Diana Serbanescu

University College London - Department of Physics and Astronomy

Nikola Ojkic

Imperial College London - Faculty of Life Sciences

Shiladitya Banerjee

University College London - Department of Physics and Astronomy

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Abstract

Cell size control emerges from a regulated balance between the rates of cell growth and division. In bacteria, simple quantitative laws connect cellular growth rate to ribosome abundance. However, it remains poorly understood how translation regulates bacterial cell size and shapes under growth perturbations. Here we develop a whole-cell model for growth dynamics in rod-shaped bacteria that links ribosomal abundance with cell geometry, division control, and the extracellular environment. Our study reveals that cell shape maintenance under nutrient perturbations requires a balanced trade-off between ribosomes and division protein synthesis. Deviations from this trade-off relationship is predicted under translational perturbations, leading to distinct modes of cell morphological changes, in agreement with single-cell experimental data on Escherichia coli. Furthermore, by calibrating our model with experimental data, we predict how combinations of nutrient-, translational- and shape perturbations can be chosen to optimize bacterial growth fitness and drug resistance.

Keywords: whole-cell model, bacterial growth, cell size, cell shape control, translation, antibiotics

Suggested Citation

Serbanescu, Diana and Ojkic, Nikola and Banerjee, Shiladitya, Nutrient-Dependent Trade-Offs between Ribosomes and Division Protein Synthesis Control Bacterial Growth and Cell Shape. CELL-REPORTS-D-20-00773. Available at SSRN: https://ssrn.com/abstract=3550210 or http://dx.doi.org/10.2139/ssrn.3550210
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Diana Serbanescu

University College London - Department of Physics and Astronomy

Gower Street
London, WC1E 6BT
United Kingdom

Nikola Ojkic

Imperial College London - Faculty of Life Sciences

United Kingdom

Shiladitya Banerjee (Contact Author)

University College London - Department of Physics and Astronomy ( email )

Gower Street
London, WC1E 6BT
United Kingdom

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