A Fast Genetically Encoded Fluorescent Sensor for Faithful
in vivo Acetylcholine Detection in Mice, Fish, Worms and Flies
Posted: 30 Mar 2020
Sneak Peek Status: Review Complete
Here we design and optimize a genetically encoded fluorescent indicator, iAChSnFR, for the ubiquitous neurotransmitter acetylcholine, based on a bacterial periplasmic binding protein. iAChSnFR shows large fluorescence changes, rapid rise and decay kinetics, and insensitivity to most cholinergic drugs. iAChSnFR revealed large transients in a variety of slice and in vivo preparations in mouse, fish, fly and worm. iAChSnFR will be useful for the study of acetylcholine in all organisms.
Keywords: acetylcholine, functional imaging, genetically encoded sensor, neurotransmitter imaging, fluorescence imaging, in vivo imaging, protein engineering, mouse, fish, Drosophila, C. elegans
Borden, Philip M. and Zhang, Peng and Shivange, Amol V and Marvin, Jonathan S. and Cichon, Joseph and Dan, Chuntao and Podgorski, Kaspar and Figueiredo, Antonio and Novak, Ondrej and Tanimoto, Masashi and Shigetomi, Eiji and Lobas, Mark A. and Kim, Hyuntae and Zhu, Paula and Zhang, Yajun and Zheng, W. Sharon and Fan, Chengcheng and Wang, Guangfu and Xiang, Bowen and Gan, Li and Zhang, Guang-Xian and Guo, Kaiming and Lin, Li and Cai, Yuan and Yee, Andrew G. and Aggarwal, Abhi and Bao, Huan and Lou, Xiaochu and Chapman, Edwin R. and Ford, Christopher P. and Rees, Douglas and Dietrich, Dirk and Khakh, Baljit S. and Dittman, Jeremy S. and Gan, Wen-Biao and Koyama, Minoru and Jayaraman, Vivek and Cheer, Joseph F. and Lester, Henry A. and Zhu, J. Julius and Looger, Loren, A Fast Genetically Encoded Fluorescent Sensor for Faithful
Acetylcholine Detection in Mice, Fish, Worms and Flies. Available at SSRN: https://ssrn.com/abstract=3554080
This is a paper under consideration at Cell Press and has not been peer-reviewed.