Viral Invasion and Type I Interferon Response Characterize the Immunophenotypes during COVID-19 Infection

27 Pages Posted: 18 Mar 2020 Last revised: 27 Mar 2020

See all articles by Lai Wei

Lai Wei

Sun Yat-Sen University (SYSU) - Zhongshan Ophthalmic Center

Siqi Ming

Sun Yat-Sen University (SYSU) - Zhongshan School of Medicine

Bin Zou

Sun Yat-Sen University (SYSU) - Zhongshan Ophthalmic Center

Yongjian Wu

the Fifth Affiliated Hospital, Sun Yat-sen University

Zhongsi Hong

Sun Yat-Sen University (SYSU) - Infectious Disease Prevention and Treatment Center

Zhonghe Li

Sun Yat-Sen University (SYSU) - Department of Nephrology

Xiaobin Zheng

Sun Yat-Sen University (SYSU) - Center for Infection and Immunity

Mingxing Huang

Sun Yat-Sen University (SYSU) - Center for Infection and Immunity

Liyun Luo

Sun Yat-Sen University (SYSU) - Center for Infection and Immunity

Juanran Liang

Sun Yat-Sen University (SYSU) - State Key Laboratory of Ophthalmology

Xiaofeng Wen

Sun Yat-Sen University (SYSU) - State Key Laboratory of Ophthalmology

Tingting Chen

Sun Yat-Sen University (SYSU) - State Key Laboratory of Ophthalmology

Qiaoxing Liang

Sun Yat-Sen University (SYSU) - State Key Laboratory of Ophthalmology

Liangjian Kuang

Sun Yat-Sen University (SYSU) - Center for Infection and Immunity

Hong Shan

Sun Yat-Sen University (SYSU) - Center for Interventional Medicine

Xi Huang

Sun Yat-Sen University (SYSU) - Center for Infection and Immunity

Multiple version iconThere are 2 versions of this paper

Date Written: March 17, 2020

Abstract

The new coronavirus COVID-19 has spread globally and caused a pneumonia outbreak. Although the whole genome sequences of COVID-19 have been rapidly obtained, pathogenesis of COVID-19 caused severe disease remains elusive. Understanding the immune responses in patients with severe disease may significantly advance the development of effective therapies. Here, using single cell RNA-seq technology, we profiled the immunophenotypes of four patients’ PBMCs before, during, and after their ICU cares. Our data identified gene expression signatures of viral invasion and type I interferon (IFN-I) responses as the key manifestations characterizing the life-threatening stage of COVID-19 infection in human. The association between viral load/IFN-α levels and disease severity in COVID-19 infection marked the key difference in the pathogenesis of this new from previous coronavirus such as SARS-CoV infections.

Note: Funding: This work was supported by Zhongshan Ophthalmic Center Annual Fund to L.W., and National Science and Technology Key Projects for Major Infectious Diseases (2017ZX10302301-002), National Natural Science Foundation of China (31470877), Guangzhou Science and Technology Planning Project (201604020006) to X.H.

Declaration of Interest: No potential conflict of interest relevant to this article was reported.

Ethical Approval: This study adhered to the tenets of the Declaration of Helsinki and was approved by the Institutional Review Boards of the Fifth Affiliated Hospital, Sun Yat-sen University (protocol # K158-1). All subjects provided written informed consent before participation of our study.

Keywords: Covid-19 pneumonia, immunophenotype, PBMC, RLRs and TLRs, type I interferon

Suggested Citation

Wei, Lai and Ming, Siqi and Zou, Bin and Wu, Yongjian and Hong, Zhongsi and Li, Zhonghe and Zheng, Xiaobin and Huang, Mingxing and Luo, Liyun and Liang, Juanran and Wen, Xiaofeng and Chen, Tingting and Liang, Qiaoxing and Kuang, Liangjian and Shan, Hong and Huang, Xi, Viral Invasion and Type I Interferon Response Characterize the Immunophenotypes during COVID-19 Infection (March 17, 2020). Available at SSRN: https://ssrn.com/abstract=3555695 or http://dx.doi.org/10.2139/ssrn.3555695

Lai Wei (Contact Author)

Sun Yat-Sen University (SYSU) - Zhongshan Ophthalmic Center ( email )

135, Xingang Xi Road
Guangzhou, Guangdong 510275
China

Siqi Ming

Sun Yat-Sen University (SYSU) - Zhongshan School of Medicine

No. 74 Zhongshan Rd. 2
Guangzhou, 510080
China

Bin Zou

Sun Yat-Sen University (SYSU) - Zhongshan Ophthalmic Center ( email )

135, Xingang Xi Road
Guangzhou, Guangdong 510275
China

Yongjian Wu

the Fifth Affiliated Hospital, Sun Yat-sen University

Zhuhai
China

Zhongsi Hong

Sun Yat-Sen University (SYSU) - Infectious Disease Prevention and Treatment Center ( email )

Zhuhai
China

Zhonghe Li

Sun Yat-Sen University (SYSU) - Department of Nephrology

Zhuhai
China

Xiaobin Zheng

Sun Yat-Sen University (SYSU) - Center for Infection and Immunity

China

Mingxing Huang

Sun Yat-Sen University (SYSU) - Center for Infection and Immunity

China

Liyun Luo

Sun Yat-Sen University (SYSU) - Center for Infection and Immunity

China

Juanran Liang

Sun Yat-Sen University (SYSU) - State Key Laboratory of Ophthalmology

135, Xingang Xi Road
Guangzhou, Guangdong 510275
China

Xiaofeng Wen

Sun Yat-Sen University (SYSU) - State Key Laboratory of Ophthalmology

135, Xingang Xi Road
Guangzhou, Guangdong 510275
China

Tingting Chen

Sun Yat-Sen University (SYSU) - State Key Laboratory of Ophthalmology

135, Xingang Xi Road
Guangzhou, Guangdong 510275
China

Qiaoxing Liang

Sun Yat-Sen University (SYSU) - State Key Laboratory of Ophthalmology

135, Xingang Xi Road
Guangzhou, Guangdong 510275
China

Liangjian Kuang

Sun Yat-Sen University (SYSU) - Center for Infection and Immunity

China

Hong Shan

Sun Yat-Sen University (SYSU) - Center for Interventional Medicine

Zhuhai
China

Xi Huang

Sun Yat-Sen University (SYSU) - Center for Infection and Immunity ( email )

China

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