Preprints with The Lancet is part of SSRN´s First Look, a place where journals identify content of interest prior to publication. Authors have opted in at submission to The Lancet family of journals to post their preprints on Preprints with The Lancet. The usual SSRN checks and a Lancet-specific check for appropriateness and transparency have been applied. Preprints available here are not Lancet publications or necessarily under review with a Lancet journal. These preprints are early stage research papers that have not been peer-reviewed. The findings should not be used for clinical or public health decision making and should not be presented to a lay audience without highlighting that they are preliminary and have not been peer-reviewed. For more information on this collaboration, see the comments published in The Lancet about the trial period, and our decision to make this a permanent offering, or visit The Lancet´s FAQ page, and for any feedback please contact firstname.lastname@example.org.
Detection of 2019-nCoV in Saliva and Characterization of Oral Symptoms in COVID-19 Patients
30 Pages Posted: 31 Mar 2020More...
Background: Coronavirus disease 19 (COVID-19) caused by a novel coronavirus (2019-nCoV) has now been classified as a pandemic by the World Health Organization (WHO). It has been reported that 2019-nCoV could be detected in various human secreta and excreta such as sputum, faeces and urine. However, the detection of 2019-nCoV in saliva has not been reported to date. To provide a more comprehensive understanding of the effects of 2019-nCoV on oral health and possible saliva transmission, the expression of the putative ACE2 (angiotensin-converting enzyme II) receptor of 2019-nCoV in salivary gland epithelial cells were analyzed in bulk RNA-seq proﬁles from public databases. Additionally, to evaluate the oral health status of COVID-19 patients, a questionnaire survey on oral-related symptoms of COVID-19 patients was performed.
Methods: To analyze ACE2 expression in salivary glands, bulk RNA-seq proﬁles from four public datasets including GTEx dataset, HPA dataset, FANTOM5 dataset and Consensus dataset were collected. Altogether, 31 COVID-19 patients were recruited, whose 2019-nCoV nucleic acid detection remained positive before or on the day of sample collection. Of these 31 patients, there were 4 critically-ill cases whereby oropharyngeal swabs were tested positive. Saliva was collected from the opening of the salivary gland canal of cleaned oral cavity to avoid contamination by other secretions from the respiratory tract. At the same time, oropharyngeal swabs were also collected. Then the presence of 2019-nCoV nucleic acids in saliva was detected by RT-PCR. Additionally, a questionnaire survey on various oral symptoms such as dry mouth and amblygeustia was also carried out on COVID-19 patients.
Findings: ACE2 expression was present at detectable levels in the salivary glands. Interestingly, we found 4 cases with positive detection of 2019-nCoV nucleic acid in saliva. It is worth noting that 3 cases with positive detection in saliva were critically-ill patients on ventilator support, thus implying a high potential (75%) for detection of 2019-nCoV in the saliva of critically-ill patients. At the same time, the two major oral-related symptoms, dry mouth (46.3%) and amblygeustia (47.2%), were manifested by a relatively high proportion of 108 COVID-19 patients.
Interpretation: This study thus confirms the expression of ACE2 in the salivary glands, and demonstrates the possibility of 2019-nCoV infection of the salivary glands. The reason that the positive saliva detection rate was as high as 75% (3/4) in critically-ill patients in our study might be due to virus invasion caused by high viral loads or destroyed salivary glands at the late stage of the disease. The emergence of viral particles in the saliva might be an indication that the disease condition of the patient has deteriorated and progressed to the terminal stage. Hence, saliva may be a new source of diagnostic specimens for critically-ill patients, since it can be easily collected without any invasive procedures.The two major oral-related symptoms, dry mouth and amblygeustia, were manifested by a relatively high proportion of COVID-19 patients suggesting that oral symptoms can also be considered as initial symptoms of COVID-19 infection.
Funding Statement: None.
Declaration of Interests: The authors declare no competing interests.
Ethics Approval Statement: This study was approved by the Medical Ethics Committee of Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China (20200062).
Keywords: 2019-nCoV, saliva, amblygeustia, dry mouth, COVID-19 patients
Suggested Citation: Suggested Citation