Pan-Cancer Analysis of m5C Regulator Genes Reveals Consistent Epigenetic Landscape Changes in Multiple Cancers
27 Pages Posted: 8 May 2020More...
Background: 5-methylcytosine (m5C) is a reversible modification to both DNA and various cellular RNAs. However, its roles in the development of human cancers are poorly understood, including the effects of mutant m5C regulators and the outcomes of modified nucleobases in RNAs.
Methods: Based on The Cancer Genome Atlas (TCGA) database, we uncovered that mutations and/or copy number variations (CNVs) of m5C regulatory genes were significantly correlated across many cancer types. We then assessed the correlation between the expression of individual m5C regulators and the activity of related hallmark pathways of cancers.
Findings: After validating the expression of m5C regulators based on their contributions to cancer development and progression, we observed their up-regulation within tumor-specific processes. Notably, our research connected aberrant alterations to m5C regulatory genes with poor clinical outcomes among various solid tumors that may drive cancer pathogenesis and/or survival.
Interpretation: We ultimately provide a landscape for the various roles of m5C regulatory genes, offering strong evidence and clinical implications for the involvement of m5C regulators across a comprehensive set of solid tumor malignancies.
Funding Statement: This study was supported by funds from the National Natural Science Foundation of China (81902832 to Y.H. and 81702927 to J.L.); the Science and Technology Research Project of Henan Province (192102310117 to Y.H. and 202102310074 to C.X.).
Declaration of Interests: The authors have no conflicts of interest to report.
Keywords: m5C regulatory genes, Frequent network mining, Pan-cancer analysis, Cancer survival
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