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Natural Cross-Linkers Stabilizedacellular Porcine Corneal Stroma for Lamellar Transplantation

56 Pages Posted: 3 Apr 2020 First Look: Under Review

See all articles by Hua Li

Hua Li

Shandong University - Department of Ophthalmology

Long Zhao

Shandong First Medical University - State Key Laboratory Cultivation Base

Fuyan Wang

Shandong University - Department of Ophthalmology

Hongwei Wang

Shandong First Medical University - State Key Laboratory Cultivation Base

Muchen Dong

Shandong First Medical University - Shandong Eye Hospital

Ting Liu

Shandong First Medical University - State Key Laboratory Cultivation Base

Ting Ruan

Shandong First Medical University - State Key Laboratory Cultivation Base

Mingming Zhou

University of Colorado, Colorado Springs - College of Business

Qingjun Zhou

Shandong First Medical University - State Key Laboratory Cultivation Base

Lixin Xie

Shandong First Medical University - State Key Laboratory Cultivation Base

Abstract

Acellular porcine corneal stroma (APCS) is a promising alternative to human donor cornea for lamellar keratoplasty (LKP). However, the detergents, enzymes and physical forces during decellularization unavoidably alter cornea extracellular matrix composition and disrupt its ultrastructure, making it less mechanically stable and liable to degradation in vitro and in vivo. Herein, we systematically analyzed the low biomechanics and easy degradability of APCS from structure and protein composition. Then, we introduced natural cross-linkers to stabilize the APCS that exhibited color variations during cross-linking. Herein, we developed a protective cross-linking system by combining cross-linkers with bovine serum albumin (BSA) to reduce color change, maintain transparency, and improve the mechanical properties of APCS. Proanthocyanidin (PA)/BSA-cross-linked APCS showed favorable corneal transparency and swelling property; the improved overall and surface corneal biomechanics were comparable to those of human donor cornea, revealing good cytocompatibility and strong resistance to enzymatic degradation. Results from corneal micropocket implantation indicated that the PA/BSA-cross-linked APCS gets more corneal stroma cells migrated and LKP in the rabbit model showed complete re-epithelialization without graft melt, and the stitches had scarcely loosened one month after the operation. The proposed method may provide more stable cornea substitutes and be applicable in other bioengineering fields.

Keywords: Acellular porcine corneal stroma, Cross-linking, Proanthocyanidin, Epigallocatechin gallate, Genipin

Suggested Citation

Li, Hua and Zhao, Long and Wang, Fuyan and Wang, Hongwei and Dong, Muchen and Liu, Ting and Ruan, Ting and Zhou, Mingming and Zhou, Qingjun and Xie, Lixin, Natural Cross-Linkers Stabilizedacellular Porcine Corneal Stroma for Lamellar Transplantation. Available at SSRN: https://ssrn.com/abstract=3560623 or http://dx.doi.org/10.2139/ssrn.3560623

Hua Li

Shandong University - Department of Ophthalmology

Jinan, 250012
China

Long Zhao

Shandong First Medical University - State Key Laboratory Cultivation Base

China

Fuyan Wang

Shandong University - Department of Ophthalmology

China

Hongwei Wang

Shandong First Medical University - State Key Laboratory Cultivation Base

China

Muchen Dong

Shandong First Medical University - Shandong Eye Hospital

China

Ting Liu

Shandong First Medical University - State Key Laboratory Cultivation Base

China

Ting Ruan

Shandong First Medical University - State Key Laboratory Cultivation Base

China

Mingming Zhou

University of Colorado, Colorado Springs - College of Business ( email )

1420 Austin Bluffs Parkway
Colorado Springs, CO 80933-7150
United States
1-719-255-5153 (Phone)

Qingjun Zhou (Contact Author)

Shandong First Medical University - State Key Laboratory Cultivation Base ( email )

China

Lixin Xie

Shandong First Medical University - State Key Laboratory Cultivation Base ( email )

China

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