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Proteomic and Metabolomic Characterization of COVID-19 Patient Sera

34 Pages Posted: 14 Apr 2020 Sneak Peek Status: Review Complete

See all articles by Bo Shen

Bo Shen

Wenzhou Medical University - Affiliated Taizhou Hospital

Xiao Yi

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

Yaoting Sun

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

Xiaojie Bi

Wenzhou Medical University - Affiliated Taizhou Hospital

Juping Du

Wenzhou Medical University - Affiliated Taizhou Hospital

Chao Zhang

Wenzhou Medical University - Affiliated Taizhou Hospital

Sheng Quan

Calibra Lab at DIAN Diagnostics

Fangfei Zhang

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

Rui Sun

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

Liujia Qian

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

Weigang Ge

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

Wei Liu

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

Shuang Liang

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

Hao Chen

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

Ying Zhang

Wenzhou Medical University - Affiliated Taizhou Hospital

Jun Li

Wenzhou Medical University - Affiliated Taizhou Hospital

Jiaqin Xu

Wenzhou Medical University - Affiliated Taizhou Hospital

Zebao He

Wenzhou Medical University - Affiliated Taizhou Hospital

Baofu Chen

Wenzhou Medical University - Affiliated Taizhou Hospital

Jing Wang

Wenzhou Medical University - Affiliated Taizhou Hospital

Haixi Yan

Wenzhou Medical University - Affiliated Taizhou Hospital

Yufen Zheng

Wenzhou Medical University - Affiliated Taizhou Hospital

Donglian Wang

Wenzhou Medical University - Affiliated Taizhou Hospital

Jiansheng Zhu

Wenzhou Medical University - Department of Infectious Diseases

Ziqing Kong

Calibra Lab at DIAN Diagnostics

Zhouyang Kang

Calibra Lab at DIAN Diagnostics

Xiao Liang

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

Xuan Ding

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

Guan Ruan

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

Nan Xiang

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

Xue Cai

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

Huanhuan Gao

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

Lu Li

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

Sainan Li

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

Qi Xiao

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

Tian Lu

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

Yi Zhu

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

Huafen Liu

Calibra Lab at DIAN Diagnostics

Haixiao Chen

Wenzhou Medical University - Affiliated Taizhou Hospital

Tiannan Guo

Westlake Institute for Advanced Study - School of Life Sciences; Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

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Abstract

Severe COVID-19 patients account for most of the mortality of this disease. Early detection and effective treatment of severe patients remain major challenges. Here, we performed proteomic and metabolomic profiling of sera from 46 COVID-19 and 53 control individuals. We then trained a machine learning model using proteomic and metabolomic measurements from a training cohort of 18 non-severe and 13 severe patients. The model correctly classified severe patients with an accuracy of 93.5%, and was further validated using ten independent patients, seven of which were correctly classified. We identified molecular changes in the sera of COVID-19 patients implicating dysregulation of macrophage, platelet degranulation and complement system pathways, and massive metabolic suppression. This study shows that it is possible to predict progression to severe COVID-19 disease using serum protein and metabolite biomarkers. Our data also uncovered molecular pathophysiology of COVID-19 with potential for developing anti-viral therapies.

Funding: This work is supported by grants from Westlake Special Program for COVID19 (2020), and Tencent foundation (2020), National Natural Science Foundation of China (81972492, 21904107, 81672086), Zhejiang Provincial Natural Science Foundation for Distinguished Young Scholars (LR19C050001), Hangzhou Agriculture and Society Advancement Program (20190101A04).

Conflict of Interest: The research group of T.G. is partly supported by Tencent, Thermo Fisher Scientific, SCIEX and Pressure Biosciences Inc. C.Z., Z.K., Z.K. and S.Q. are employees of DIAN Diagnostics.

Keywords: COVID-19, Proteomics, metabolomics, serum

Suggested Citation

Shen, Bo and Yi, Xiao and Sun, Yaoting and Bi, Xiaojie and Du, Juping and Zhang, Chao and Quan, Sheng and Zhang, Fangfei and Sun, Rui and Qian, Liujia and Ge, Weigang and Liu, Wei and Liang, Shuang and Chen, Hao and Zhang, Ying and Li, Jun and Xu, Jiaqin and He, Zebao and Chen, Baofu and Wang, Jing and Yan, Haixi and Zheng, Yufen and Wang, Donglian and Zhu, Jiansheng and Kong, Ziqing and Kang, Zhouyang and Liang, Xiao and Ding, Xuan and Ruan, Guan and Xiang, Nan and Cai, Xue and Gao, Huanhuan and Li, Lu and Li, Sainan and Xiao, Qi and Lu, Tian and Zhu, Yi and Liu, Huafen and Chen, Haixiao and Guo, Tiannan, Proteomic and Metabolomic Characterization of COVID-19 Patient Sera. Available at SSRN: https://ssrn.com/abstract=3570565 or http://dx.doi.org/10.2139/ssrn.3570565
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Bo Shen

Wenzhou Medical University - Affiliated Taizhou Hospital

Taizhou
China

Xiao Yi

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

18 Shilongshan Road
Hangzhou, Zhejiang Province 310024
China

Yaoting Sun

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

18 Shilongshan Road
Hangzhou, Zhejiang Province 310024
China

Xiaojie Bi

Wenzhou Medical University - Affiliated Taizhou Hospital

Taizhou
China

Juping Du

Wenzhou Medical University - Affiliated Taizhou Hospital

Taizhou
China

Chao Zhang

Wenzhou Medical University - Affiliated Taizhou Hospital

Taizhou
China

Sheng Quan

Calibra Lab at DIAN Diagnostics

China

Fangfei Zhang

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

18 Shilongshan Road
Hangzhou, Zhejiang Province 310024
China

Rui Sun

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

18 Shilongshan Road
Hangzhou, Zhejiang Province 310024
China

Liujia Qian

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

18 Shilongshan Road
Hangzhou, Zhejiang Province 310024
China

Weigang Ge

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

18 Shilongshan Road
Hangzhou, Zhejiang Province 310024
China

Wei Liu

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

18 Shilongshan Road
Hangzhou, Zhejiang Province 310024
China

Shuang Liang

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

18 Shilongshan Road
Hangzhou, Zhejiang Province 310024
China

Hao Chen

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

18 Shilongshan Road
Hangzhou, Zhejiang Province 310024
China

Ying Zhang

Wenzhou Medical University - Affiliated Taizhou Hospital

Taizhou
China

Jun Li

Wenzhou Medical University - Affiliated Taizhou Hospital

Taizhou
China

Jiaqin Xu

Wenzhou Medical University - Affiliated Taizhou Hospital

Taizhou
China

Zebao He

Wenzhou Medical University - Affiliated Taizhou Hospital

Taizhou
China

Baofu Chen

Wenzhou Medical University - Affiliated Taizhou Hospital ( email )

Taizhou
China

Jing Wang

Wenzhou Medical University - Affiliated Taizhou Hospital

Taizhou
China

Haixi Yan

Wenzhou Medical University - Affiliated Taizhou Hospital

Taizhou
China

Yufen Zheng

Wenzhou Medical University - Affiliated Taizhou Hospital

Taizhou
China

Donglian Wang

Wenzhou Medical University - Affiliated Taizhou Hospital

Taizhou
China

Jiansheng Zhu

Wenzhou Medical University - Department of Infectious Diseases ( email )

150 Ximen Road of Linhai City
Taizhou
China

Ziqing Kong

Calibra Lab at DIAN Diagnostics

China

Zhouyang Kang

Calibra Lab at DIAN Diagnostics

China

Xiao Liang

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

18 Shilongshan Road
Hangzhou, Zhejiang Province 310024
China

Xuan Ding

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

18 Shilongshan Road
Hangzhou, Zhejiang Province 310024
China

Guan Ruan

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

18 Shilongshan Road
Hangzhou, Zhejiang Province 310024
China

Nan Xiang

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

18 Shilongshan Road
Hangzhou, Zhejiang Province 310024
China

Xue Cai

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

18 Shilongshan Road
Hangzhou, Zhejiang Province 310024
China

Huanhuan Gao

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

18 Shilongshan Road
Hangzhou, Zhejiang Province 310024
China

Lu Li

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

18 Shilongshan Road
Hangzhou, Zhejiang Province 310024
China

Sainan Li

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

18 Shilongshan Road
Hangzhou, Zhejiang Province 310024
China

Qi Xiao

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

18 Shilongshan Road
Hangzhou, Zhejiang Province 310024
China

Tian Lu

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences

18 Shilongshan Road
Hangzhou, Zhejiang Province 310024
China

Yi Zhu

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences ( email )

18 Shilongshan Road
Hangzhou, Zhejiang Province 310024
China

Huafen Liu

Calibra Lab at DIAN Diagnostics

China

Haixiao Chen

Wenzhou Medical University - Affiliated Taizhou Hospital ( email )

Taizhou
China

Tiannan Guo (Contact Author)

Westlake Institute for Advanced Study - School of Life Sciences ( email )

18 Shilongshan Road
Hangzhou, Zhejiang Province 310024
China

Westlake Institute for Advanced Study - Institute of Basic Medical Sciences ( email )

18 Shilongshan Road
Hangzhou, Zhejiang Province 310024
China

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