Genome-Wide Screens Identify Lineage- and Tumor-Specific Genes Modulating MHC-I and MHC-II Immunosurveillance in Human Lymphomas
39 Pages Posted: 14 May 2020 Sneak Peek Status: Under ReviewMore...
Tumors frequently subvert MHC class I (MHC-I) peptide presentation to evade CD8+ T cell immunosurveillance. To better define the regulatory networks controlling antigen presentation, we employed genome-wide screening in human diffuse large B cell lymphomas (DLBCLs). This approach revealed dozens of novel genes that positively and negatively modulate MHC-I cell surface levels. Identified genes cluster in multiple pathways including cytokine signaling, mRNA processing, endosomal trafficking, and protein metabolism. Many genes exhibit lymphoma subtype- or tumor-specific MHC-I regulation, and a majority of primary DLBCL tumors display genetic alterations in multiple regulators. We establish that the HSP90 co-chaperone SUGT1 is a major positive regulator of both MHC-I and MHC-II cell surface expression. Further, pharmacological inhibition of two negative regulators of antigen presentation, EZH2 and thymidylate synthase, enhances DLBCL MHC-I presentation. These and other genes represent potential targets for manipulating MHC-I immunosurveillance in cancers, infectious diseases, and autoimmunity.
Keywords: diffuse large B-cell lymphoma, antigen presentation, MHC class I, MHC-I, MHC class II, Immunotherapy, immunoevasion, EZH2, SUGT1, thymidylate synthase
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