puc-header

Regulation of Calcium Oscillations in ß-Cells by Co-Activated Cannabinoid Receptors

21 Pages Posted: 19 May 2020 Publication Status: Review Complete

See all articles by Aurélien Laguerre

Aurélien Laguerre

Oregon Health and Science University - Department of Chemical Physiology & Biochemistry

Kaya Keutler

Oregon Health and Science University - Department of Chemical Physiology & Biochemistry

Sebastian Hauke

Oregon Health and Science University - Department of Chemical Physiology & Biochemistry

Carsten Schultz

European Molecular Biology Laboratory (EMBL)- Germany - Cell Biology and Biophysics Unit

More...

Abstract

Pharmacological treatment of pancreatic β-cells targeting cannabinoid receptor 1 and 2 (CB1 and CB2) has been shown to result in significant effects on insulin release, possibly by modulating intracellular calcium levels ([Ca2+]i). It is unclear how the interplay of CB1 and CB2 affects insulin secretion. Here, we demonstrate by the use of highly specific receptor antagonists and the newly developed photo-releasable endocannabinoid 2-arachidonoylgylcerol (caged 2-AG, cg2-AG) that both receptors have counteracting effects on cytosolic calcium oscillations. We further show that both receptors are juxtaposed in a way that increases ([Ca2+]i) oscillations in silent β-cells but dampens them in active ones. This study highlights a new functional role of cannabinoid receptors 1 and 2 acting in concert as a compensator/attenuator switch for regulating β-cell excitability.

Keywords: signal transduction, insulin secretion, pancreatic islets, caged compounds, calcium oscillations

Suggested Citation

Laguerre, Aurélien and Keutler, Kaya and Hauke, Sebastian and Schultz, Carsten, Regulation of Calcium Oscillations in ß-Cells by Co-Activated Cannabinoid Receptors. Available at SSRN: https://ssrn.com/abstract=3587002 or http://dx.doi.org/10.2139/ssrn.3587002
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Aurélien Laguerre

Oregon Health and Science University - Department of Chemical Physiology & Biochemistry

3181 S.W. Sam Jackson Park Rd.
Portland, OR 97201
United States

Kaya Keutler

Oregon Health and Science University - Department of Chemical Physiology & Biochemistry

3181 S.W. Sam Jackson Park Rd.
Portland, OR 97201
United States

Sebastian Hauke

Oregon Health and Science University - Department of Chemical Physiology & Biochemistry

3181 S.W. Sam Jackson Park Rd.
Portland, OR 97201
United States

Carsten Schultz (Contact Author)

European Molecular Biology Laboratory (EMBL)- Germany - Cell Biology and Biophysics Unit ( email )

Meyerhofstraße 1
Heidelberg, 69117
Germany

Click here to go to Cell.com

Paper statistics

Abstract Views
213
Downloads
7
PlumX Metrics