Raman Microspectroscopy and Imaging Reveal Novel Biomarkers Specific for Thoracic Aortic Aneurysms
58 Pages Posted: 10 Jun 2020 Publication Status: PublishedMore...
Aortic rupture and dissection are life-threatening complications of ascending thoracic aortic aneurysms (aTAAs) and the risk assessment has been largely based on the monitoring of a lumen size enlargement. Temporal changes of the extracellular matrix (ECM), which has a critical influence on aortic remodeling, are not routinely evaluated nor cardiovascular biomarkers exist to predict aTAA formation. Here, we performed Raman microspectroscopy and imaging and identified marker spectra specific for aTAAs in mice and humans by multivariate data analysis (MVA). Multivariate curve resolution-alternating least squares (MCR-ALS) combined with Lasso regression successfully identified elastic fiber-derived Ce1 and collagen fiber-derived Cc6 components, which were significantly increased in aTAA lesions of murine and human aortic tissues. Cc6 detected the changes in amino acid residues, including phenylalanine, tyrosine, tryptophan, cysteine, aspartate, and glutamate. Ce1 and Cc6 may serve as a diagnostic Raman spectral marker that detects alterations of amino acids derived from aneurysm lesions.
Keywords: Ascending thoracic aortic aneurysm, label-free Raman imaging, novel biomarker, extracellular matrix, multivariate data analysis, collagen fibers, elastic fibers
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