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Raman Microspectroscopy and Imaging Reveal Novel Biomarkers Specific for Thoracic Aortic Aneurysms

58 Pages Posted: 10 Jun 2020 Publication Status: Published

See all articles by Kaori Sugiyama

Kaori Sugiyama

University of Tsukuba - Life Science Center for Survival Dynamics

Julia Marzi

Eberhard Karls University - Department of Women’s Health

Eva Maria Brauchle

Eberhard Karls University - Department of Women’s Health

Masahiro Ando

Waseda University - Research Organization for Nano & Life Innovation

Yoshito Yamashiro

University of Tsukuba - Life Science Center for Survival Dynamics

Bhama Ramkhelawon

New York University (NYU) - New York University

Katja Schenke-Layland

University of Tübingen - NMI Natural and Medical Sciences Institute; Eberhard Karls University of Tübingen - Department of Women’s Health

Hiromi Yanagisawa

University of Tsukuba - Life Science Center for Survival Dynamics

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Abstract

Aortic rupture and dissection are life-threatening complications of ascending thoracic aortic aneurysms (aTAAs) and the risk assessment has been largely based on the monitoring of a lumen size enlargement. Temporal changes of the extracellular matrix (ECM), which has a critical influence on aortic remodeling, are not routinely evaluated nor cardiovascular biomarkers exist to predict aTAA formation. Here, we performed Raman microspectroscopy and imaging and identified marker spectra specific for aTAAs in mice and humans by multivariate data analysis (MVA). Multivariate curve resolution-alternating least squares (MCR-ALS) combined with Lasso regression successfully identified elastic fiber-derived Ce1 and collagen fiber-derived Cc6 components, which were significantly increased in aTAA lesions of murine and human aortic tissues. Cc6 detected the changes in amino acid residues, including phenylalanine, tyrosine, tryptophan, cysteine, aspartate, and glutamate. Ce1 and Cc6 may serve as a diagnostic Raman spectral marker that detects alterations of amino acids derived from aneurysm lesions.

Keywords: Ascending thoracic aortic aneurysm, label-free Raman imaging, novel biomarker, extracellular matrix, multivariate data analysis, collagen fibers, elastic fibers

Suggested Citation

Sugiyama, Kaori and Marzi, Julia and Brauchle, Eva Maria and Ando, Masahiro and Yamashiro, Yoshito and Ramkhelawon, Bhama and Schenke-Layland, Katja and Yanagisawa, Hiromi, Raman Microspectroscopy and Imaging Reveal Novel Biomarkers Specific for Thoracic Aortic Aneurysms. Available at SSRN: https://ssrn.com/abstract=3606775 or http://dx.doi.org/10.2139/ssrn.3606775
This version of the paper has not been formally peer reviewed.

Kaori Sugiyama

University of Tsukuba - Life Science Center for Survival Dynamics

Tsukuba University , Ibaraki Ken
Tsukuba, Ibaraki 305-8573, 3050006
Japan

Julia Marzi

Eberhard Karls University - Department of Women’s Health ( email )

Germany

Eva Maria Brauchle

Eberhard Karls University - Department of Women’s Health ( email )

Germany

Masahiro Ando

Waseda University - Research Organization for Nano & Life Innovation

1-104 Totsukamachi, Shinjuku-ku
tokyo, 169-8050
Japan

Yoshito Yamashiro

University of Tsukuba - Life Science Center for Survival Dynamics ( email )

1-1-1 Tennodai
Tsukuba, Ibaraki 305-8575
Japan

Bhama Ramkhelawon

New York University (NYU) - New York University

Katja Schenke-Layland

University of Tübingen - NMI Natural and Medical Sciences Institute ( email )

Reutlingen
Germany

Eberhard Karls University of Tübingen - Department of Women’s Health ( email )

Germany

Hiromi Yanagisawa (Contact Author)

University of Tsukuba - Life Science Center for Survival Dynamics

1-1-1 Tennodai
Tsukuba, Ibaraki 305-8575
Japan

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