3D-Bioprinted Human Periodontal/Osteoblastic Microfluidic Tissue-on-A-Chip System and Evaluation of Model Drug Interactions

42 Pages Posted: 19 Jun 2020

See all articles by Murat Taner Vurat

Murat Taner Vurat

Ankara University - Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory

Şükran Şeker

Ankara University - Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory

Özge Lalegül-Ülker

Ankara University - Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory

Mahmut Parmaksiz

Ankara University - Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory

Ayşe Eser Elçin

Ankara University - Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory

Yaşar Murat Elçin

Ankara University - Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory

Abstract

While periodontal (PD) disease is among principal causes of tooth loss worldwide, regulation of concomitant soft and mineralized PD tissues, and PD pathogenesis have not been completely clarified yet. Besides, relevant pre-clinical models and in vitro platforms have limitations in accurately simulating human physiology. A periodontium-on-a-chip, emulating PD ligament-alveolar bone (PDL-AB) biointerface has not been developed to date. In this study, we have harnessed three-dimensional bioprinting (3DP) technology for developing such a system for the first time. PDL was modelled by using gelatin methacryloyl (Gel-MA) bioink for bioprinting human periodontal ligament fibroblasts. On the side, AB was modelled by using a composite bioink comprised of Gel-MA and hydroxyapatite-magnetic iron oxide nanoparticles (Gel-MA/HAp-MNPs) for bioprinting human osteoblasts. MNPs were incorporated to bring magnetic properties to the mineralized layer for use in prospective orthodontic mechanotransduction studies. The 3DP parameters of the composite microtissue were optimized; the physical, chemical, rheological, mechanical, and thermal properties of the constructs were assessed. Finally, preliminary evaluation of the model drug tetracycline interactions was performed. Thus, the effects of the tetracyclines on PDL-AB have clinical significance for treating PD diseases. This periodontium-on-a-chip model represents a reproducible in vitro platform for studying processes of healthy and diseased human PDL.

Keywords: Periodontium-on-a-chip, 3D bioprinting, Periodontal-osteoblastic biointerface, Periodontal ligament, Alveolar bone, Organ-on-a-chip, Tissue modeling, Tetracycline absorption, Cytotoxicity, Composite microtissue

Suggested Citation

Vurat, Murat Taner and Şeker, Şükran and Lalegül-Ülker, Özge and Parmaksiz, Mahmut and Elçin, Ayşe Eser and Elçin, Yaşar Murat, 3D-Bioprinted Human Periodontal/Osteoblastic Microfluidic Tissue-on-A-Chip System and Evaluation of Model Drug Interactions. Available at SSRN: https://ssrn.com/abstract=3622631 or http://dx.doi.org/10.2139/ssrn.3622631

Murat Taner Vurat (Contact Author)

Ankara University - Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory

Ankara
Turkey

Şükran Şeker

Ankara University - Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory

Ankara
Turkey

Özge Lalegül-Ülker

Ankara University - Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory

Ankara
Turkey

Mahmut Parmaksiz

Ankara University - Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory

Ankara
Turkey

Ayşe Eser Elçin

Ankara University - Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory ( email )

Ankara
Turkey

Yaşar Murat Elçin

Ankara University - Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory

Ankara
Turkey

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