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An Injectable Click-Crosslinked Hyaluronic Acid Hydrogel Modified with a BMP-2 Mimic Peptide as a Bone Tissue Engineering Scaffold

46 Pages Posted: 19 Jun 2020 Publication Status: Under Review

See all articles by Seung Hun Park

Seung Hun Park

Ajou University - Department of Molecular Science and Technology

Joon Yeong Park

Ajou University - Department of Molecular Science and Technology

Yun Bae Ji

Ajou University - Department of Molecular Science and Technology

Hyun Jin Ju

Ajou University - Department of Molecular Science and Technology

Byoung Hyun Min

Ajou University - Department of Molecular Science and Technology

Moon Suk Kim

Ajou University - Department of Molecular Science and Technology

Abstract

An injectable, click-crosslinking (Cx) hyaluronic acid (HA) hydrogel scaffold modified with a bone morphogenetic protein-2 (BMP-2) mimetic peptide (BP) was prepared for bone tissue engineering applications. The injectable click-crosslinking HA formulation was prepared from HA-tetrazine (HA-Tet) and HA-cyclooctene (HA-TCO). The Cx-HA hydrogel scaffold was prepared simply by mixing HA-Tet and HA-TCO. The Cx-HA hydrogel scaffold was stable for a longer period than HA both in vitro and in vivo, which was verified via in-vivo fluorescence imaging in real time. BP acted as an osteogenic differentiation factor for human dental pulp stem cells (hDPSCs). After its formation in vivo, the Cx-HA scaffold provided an excellent environment for the hDPSCs, and the biocompatibility of the hydrogel scaffold with tissue was excellent. Like traditional BMP-2, BP induced the osteogenic differentiation of hDPSCs in vitro. The physical properties and injectability of the chemically loaded BP for the Cx-HA hydrogel (Cx-HA-BP) were nearly identical to those of the physically loaded BP hydrogels and the Cx-HA-BP formulation quickly formed a hydrogel scaffold in vivo. The chemically loaded hydrogel scaffold retained the BP for over a month. The Cx-HA-BP hydrogel was better at inducing the osteogenic differentiation of loaded hDPSCs, because it prolonged the availability of BP. In summary, we successfully developed an injectable, click-crosslinking Cx-HA hydrogel scaffold to prolong the availability of BP for efficient bone tissue engineering.

Keywords: BMP-2 mimetic peptide, bone tissue engineering, injectable hydrogel scaffold, click-crosslinking, human dental pulp stem cells, osteogenic differentiation

Suggested Citation

Park, Seung Hun and Park, Joon Yeong and Ji, Yun Bae and Ju, Hyun Jin and Min, Byoung Hyun and Kim, Moon Suk, An Injectable Click-Crosslinked Hyaluronic Acid Hydrogel Modified with a BMP-2 Mimic Peptide as a Bone Tissue Engineering Scaffold. Available at SSRN: https://ssrn.com/abstract=3622648 or http://dx.doi.org/10.2139/ssrn.3622648

Seung Hun Park (Contact Author)

Ajou University - Department of Molecular Science and Technology

Korea, Republic of (South Korea)

Joon Yeong Park

Ajou University - Department of Molecular Science and Technology

Korea, Republic of (South Korea)

Yun Bae Ji

Ajou University - Department of Molecular Science and Technology

Korea, Republic of (South Korea)

Hyun Jin Ju

Ajou University - Department of Molecular Science and Technology

Korea, Republic of (South Korea)

Byoung Hyun Min

Ajou University - Department of Molecular Science and Technology

Korea, Republic of (South Korea)

Moon Suk Kim

Ajou University - Department of Molecular Science and Technology ( email )

Korea, Republic of (South Korea)

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