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CD27 hiCD38 hi Plasmablasts are Activated B Cells of Mixed Origin with Distinct Function

48 Pages Posted: 8 Jul 2020 Publication Status: Review Complete

See all articles by Angeline Rouers

Angeline Rouers

Agency for Science, Technology and Research (A*STAR) - A*STAR Infectious Diseases Labs

Ramapraba Appanna

Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN)

Marion Chevrier

Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN)

Josephine Lum

Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN)

MaiChan Lau

Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN)

Lingqiao Tan

Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN)

Alicia Tay

Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN)

Durgalakshmi Sathiakumar

Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN)

Kaval Kaur

Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN)

Julia Boehme

Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN)

Yee-Sin Leo

National University of Singapore (NUS) - Department of Microbiology and Immunology

Shanshan W. Howland

Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN)

Amit Singhal

Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN)

Jinmiao Chen

Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN)

Katja Fink

Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN)

More...

Abstract

Clinically important broadly reactive B cells evolve during multiple infections. The phenotype and function of B cells re-activated during secondary infection is different compared to B cells activated after a primary infection. Here we studied CD27high CD38high plasmablasts from patients with a primary or secondary dengue virus infection. Three transcriptionally and functionally distinct plasmablast clusters were identified. The largest cluster 0/1 was plasma cell-related and contained cells coding for virus serotype cross-reactive antibodies of the IgG1 isotype, consistent with memory B cell activation during an extrafollicular response. In contrast, cells in clusters 2 and 3 expressed low levels of antibody genes and high levels of genes associated with oxidative phosphorylation, EIF2 pathway and mitochondrial dysfunction. Clusters 2 and 3 also showed a transcriptional footprint of T cell help, in line with activation from either na├»ve B cells or memory B cells in the context of a germinal center reaction.

Keywords: plasmablasts, viral infection, antibodies, single cell RNAseq, dengue, B cell memory, antibody-secreting cells

Suggested Citation

Rouers, Angeline and Appanna, Ramapraba and Chevrier, Marion and Lum, Josephine and Lau, MaiChan and Tan, Lingqiao and Tay, Alicia and Sathiakumar, Durgalakshmi and Kaur, Kaval and Boehme, Julia and Leo, Yee-Sin and Howland, Shanshan W. and Singhal, Amit and Chen, Jinmiao and Fink, Katja, CD27 hiCD38 hi Plasmablasts are Activated B Cells of Mixed Origin with Distinct Function. Available at SSRN: https://ssrn.com/abstract=3631403 or http://dx.doi.org/10.2139/ssrn.3631403
This version of the paper has not been formally peer reviewed.

Angeline Rouers

Agency for Science, Technology and Research (A*STAR) - A*STAR Infectious Diseases Labs ( email )

Singapore

Ramapraba Appanna

Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN) ( email )

1 Fusionopolis Way
#16-16 Connexis
Singapore, 138632
Singapore

Marion Chevrier

Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN) ( email )

1 Fusionopolis Way
#16-16 Connexis
Singapore, 138632
Singapore

Josephine Lum

Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN)

1 Fusionopolis Way
#16-16 Connexis
Singapore, 138632
Singapore

MaiChan Lau

Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN) ( email )

1 Fusionopolis Way
#16-16 Connexis
Singapore, 138632
Singapore

Lingqiao Tan

Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN) ( email )

1 Fusionopolis Way
#16-16 Connexis
Singapore, 138632
Singapore

Alicia Tay

Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN) ( email )

1 Fusionopolis Way
#16-16 Connexis
Singapore, 138632
Singapore

Durgalakshmi Sathiakumar

Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN) ( email )

1 Fusionopolis Way
#16-16 Connexis
Singapore, 138632
Singapore

Kaval Kaur

Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN) ( email )

1 Fusionopolis Way
#16-16 Connexis
Singapore, 138632
Singapore

Julia Boehme

Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN) ( email )

1 Fusionopolis Way
#16-16 Connexis
Singapore, 138632
Singapore

Yee-Sin Leo

National University of Singapore (NUS) - Department of Microbiology and Immunology ( email )

5 Science Drive 2 Blk MD4, Level 3
117545
Singapore

Shanshan W. Howland

Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN) ( email )

1 Fusionopolis Way
#16-16 Connexis
Singapore, 138632
Singapore

Amit Singhal

Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN) ( email )

1 Fusionopolis Way
#16-16 Connexis
Singapore, 138632
Singapore

Jinmiao Chen

Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN)

1 Fusionopolis Way
#16-16 Connexis
Singapore, 138632
Singapore

Katja Fink (Contact Author)

Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN) ( email )

1 Fusionopolis Way
#16-16 Connexis
Singapore, 138632
Singapore

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