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Multivalent Clustering of Adhesion Ligands in Nanofiber-Nanoparticle Composites

33 Pages Posted: 13 Jul 2020 Publication Status: Accepted

See all articles by Dounia Dems

Dounia Dems

Sorbonne Université, CNRS, Collège de France, Laboratoire de Chimie de la Matière Condensée de Paris

Ronit Freeman

Simpson Querrey Institute, Northwestern University

Thibaud Coradin

Sorbonne Université, CNRS, Collège de France, Laboratoire de Chimie de la Matière Condensée de Paris

Samuel I. Stupp

Simpson Querrey Institute, Northwestern University

Carole Aime

Université Paris I Panthéon-Sorbonne - Laboratoire de Chimie de la Matière Condensée de Paris

Abstract

Because the positioning and clustering of biomolecules within the extracellular matrix dictates cell behaviors, the engineering of biomaterials incorporating bioactive epitopes with spatial organization tunable at the nanoscale is of primary importance. Here we used a highly modular composite approach combining peptide amphiphile (PA) nanofibers and silica nanoparticles, which are both easily functionalized with one or several bioactive signals. We show that the surface of silica nanoparticles allows the clustering of RGDS bioactive signals leading to improved adhesion and spreading of fibroblast cells on composite hydrogels at an epitope concentration much lower than in PA-only based matrices. Most importantly, by combining the two integrin-binding sequences RGDS and PHSRN on nanoparticle surfaces, we improved cell adhesion on the PA nanofiber/particle composite hydrogels, which is attributed to synergistic interactions known to be effective only for peptide intermolecular distance of ca. 5 nm. Such composites with soft and hard nanostructures offer a strategy for the design of advanced scaffolds to display multiple signals and control cell behavior.

Suggested Citation

Dems, Dounia and Freeman, Ronit and Coradin, Thibaud and Stupp, Samuel I. and Aime, Carole, Multivalent Clustering of Adhesion Ligands in Nanofiber-Nanoparticle Composites. Available at SSRN: https://ssrn.com/abstract=3640709 or http://dx.doi.org/10.2139/ssrn.3640709

Dounia Dems

Sorbonne Université, CNRS, Collège de France, Laboratoire de Chimie de la Matière Condensée de Paris

Ronit Freeman

Simpson Querrey Institute, Northwestern University

Thibaud Coradin

Sorbonne Université, CNRS, Collège de France, Laboratoire de Chimie de la Matière Condensée de Paris

Samuel I. Stupp

Simpson Querrey Institute, Northwestern University

Carole Aime (Contact Author)

Université Paris I Panthéon-Sorbonne - Laboratoire de Chimie de la Matière Condensée de Paris ( email )

Paris
France

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