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SARS-CoV-2 Infection Dysregulates the Metabolomic and Lipidomic Profiles of Serum

28 Pages Posted: 7 Aug 2020 Publication Status: Review Complete

See all articles by Chiara Bruzzone

Chiara Bruzzone

Basque Research and Technology Alliance (BRTA) - Precision Medicine and Metabolism Laboratory

Maider Bizkarguenaga

Basque Research and Technology Alliance (BRTA) - Precision Medicine and Metabolism Laboratory

Rubén Gil-Redondo

Basque Research and Technology Alliance (BRTA) - Precision Medicine and Metabolism Laboratory

Tammo Diercks

Basque Research and Technology Alliance - NMR Platform

Eunate Arana

University of the Basque Country - Biocruces Bizkaia Health Research Institute

Aitor García de Vicuña

Cruces University Hospital - Biocruces Bizkaia Health Research Institute

Marisa Seco

OSARTEN Kooperativa Elkarte

Alexandre Bosch

Basque Research and Technology Alliance - Cancer Immunology and Immunotherapy Lab

Asís Palazón

Basque Research and Technology Alliance - Cancer Immunology and Immunotherapy Lab

Itxaso San Juan

Basque Research and Technology Alliance (BRTA) - Precision Medicine and Metabolism Laboratory

Ana Laín

Basque Research and Technology Alliance (BRTA) - Precision Medicine and Metabolism Laboratory

Jon Gil-Martínez

Basque Research and Technology Alliance (BRTA) - Precision Medicine and Metabolism Laboratory

Ganeko Bernardo-Seisdedos

ATLAS Molecular Pharma S. L.

David Fernandez-RaMOS

Basque Research and Technology Alliance (BRTA) - Precision Medicine and Metabolism Laboratory

Fernando Lopitz-Otsoa

Basque Research and Technology Alliance (BRTA) - Precision Medicine and Metabolism Laboratory

Nieves Embade

Basque Research and Technology Alliance (BRTA) - Precision Medicine and Metabolism Laboratory

Shelly C. Lu

Cedars Sinai Medical Center - Division of Digestive and Liver Diseases

José M. Mato

Basque Research and Technology Alliance (BRTA) - Precision Medicine and Metabolism Laboratory

Oscar Millet

Basque Research and Technology Alliance (BRTA) - Precision Medicine and Metabolism Laboratory

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Abstract

Coronavirus disease (COVID‐19) is a systemic infection that exerts significant impact on the metabolism. Yet, there is little information on how SARS-CoV-2, the virus causing COVID-19, affects metabolism. Using NMR spectroscopy, we measured the metabolomic and lipidomic serum profile from 263 symptomatic patients hospitalized after positive PCR testing for SARS-CoV-2 infection. We also established the profiles of 280 persons collected before the coronavirus pandemic started. PCA analyses discriminated both cohorts, highlighting the impact that the infection has in overall metabolism. The lipidomic analysis unraveled a pathogenic redistribution of the lipoprotein particle size and composition to increase the atherosclerotic risk. In turn, metabolomic analysis reveals abnormally high levels of ketone bodies (acetoacetic acid, 3-hydroxybutyric acid and acetone) and 2-hydroxybutyric acid, a readout of hepatic glutathione synthesis and marker of oxidative stress. Our results are consistent with a model in which SARS-CoV-2 infection induces liver damage associated with dyslipidemia and oxidative stress.

Ethical Approval: All serum samples were provided by the Basque Biobank for research (BIOEF). According to the Declaration of Helsinki principles, all participants in the study provided informed consent to clinical investigations, with evaluation and approval from the corresponding ethics committee (CEIC-E 20-26, 1-2016). All data was anonymized to protect the confidentiality of participants.

Keywords: COVID-19, metabolomics, lipidomics, NMR spectroscopy, diabetic ketoacidosis, infectious disease

Suggested Citation

Bruzzone, Chiara and Bizkarguenaga, Maider and Gil-Redondo, Rubén and Diercks, Tammo and Arana, Eunate and García de Vicuña, Aitor and Seco, Marisa and Bosch, Alexandre and Palazón, Asís and San Juan, Itxaso and Laín, Ana and Gil-Martínez, Jon and Bernardo-Seisdedos, Ganeko and Fernandez-RaMOS, David and Lopitz-Otsoa, Fernando and Embade, Nieves and Lu, Shelly C. and Mato, José M. and Millet, Oscar, SARS-CoV-2 Infection Dysregulates the Metabolomic and Lipidomic Profiles of Serum. Available at SSRN: https://ssrn.com/abstract=3648224 or http://dx.doi.org/10.2139/ssrn.3648224
This version of the paper has not been formally peer reviewed.

Chiara Bruzzone

Basque Research and Technology Alliance (BRTA) - Precision Medicine and Metabolism Laboratory ( email )

Spain

Maider Bizkarguenaga

Basque Research and Technology Alliance (BRTA) - Precision Medicine and Metabolism Laboratory ( email )

Spain

Rubén Gil-Redondo

Basque Research and Technology Alliance (BRTA) - Precision Medicine and Metabolism Laboratory ( email )

Spain

Tammo Diercks

Basque Research and Technology Alliance - NMR Platform ( email )

Spain

Eunate Arana

University of the Basque Country - Biocruces Bizkaia Health Research Institute ( email )

Spain

Aitor García de Vicuña

Cruces University Hospital - Biocruces Bizkaia Health Research Institute ( email )

Marisa Seco

OSARTEN Kooperativa Elkarte ( email )

Alexandre Bosch

Basque Research and Technology Alliance - Cancer Immunology and Immunotherapy Lab ( email )

Spain

Asís Palazón

Basque Research and Technology Alliance - Cancer Immunology and Immunotherapy Lab ( email )

Itxaso San Juan

Basque Research and Technology Alliance (BRTA) - Precision Medicine and Metabolism Laboratory ( email )

Spain

Ana Laín

Basque Research and Technology Alliance (BRTA) - Precision Medicine and Metabolism Laboratory ( email )

Spain

Jon Gil-Martínez

Basque Research and Technology Alliance (BRTA) - Precision Medicine and Metabolism Laboratory ( email )

Spain

Ganeko Bernardo-Seisdedos

ATLAS Molecular Pharma S. L. ( email )

David Fernandez-RaMOS

Basque Research and Technology Alliance (BRTA) - Precision Medicine and Metabolism Laboratory ( email )

Spain

Fernando Lopitz-Otsoa

Basque Research and Technology Alliance (BRTA) - Precision Medicine and Metabolism Laboratory

Spain

Nieves Embade

Basque Research and Technology Alliance (BRTA) - Precision Medicine and Metabolism Laboratory ( email )

Spain

Shelly C. Lu

Cedars Sinai Medical Center - Division of Digestive and Liver Diseases

8700 Beverly Blvd.
Los Angeles, CA 90048
United States

José M. Mato

Basque Research and Technology Alliance (BRTA) - Precision Medicine and Metabolism Laboratory

Spain

Oscar Millet (Contact Author)

Basque Research and Technology Alliance (BRTA) - Precision Medicine and Metabolism Laboratory ( email )

Spain

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