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The Importance of Earth Reference Controls in Spaceflight – Omics Experiments: Dissecting Transcriptional Responses of Nucleolin Mutants to Red Light Stimulation

32 Pages Posted: 11 Aug 2020 Publication Status: Published

See all articles by Aránzazu Manzano

Aránzazu Manzano

Centro de Investigaciones Biológicas Margarita Salas (CSIC)

Alicia Villacampa

Centro de Investigaciones Biológicas Margarita Salas (CSIC)

Julio Sáez-Vásquez

CNRS - , Laboratoire Génome et Développement des Plantes (LGDP)

John Z. Kiss

University of North Carolina (UNC) at Greensboro - Department of Biology

F. Javier Medina

Centro de Investigaciones Biológicas Margarita Salas (CSIC)

Raul Herranz

Centro de Investigaciones Biológicas Margarita Salas (CSIC)

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Abstract

Understanding plant adaptive responses to the space environment is a requisite for enabling space farming. Spaceflight produces deleterious effects on plant cells, particularly affecting ribosome biogenesis, a complex stress-sensitive process coordinated with cell division and differentiation, known to be activated by red light. Here, we have used mutants from the two nucleolin genes in Arabidopsis (NUC1 and NUC2), encoding the main regulator of the ribosome biogenesis in the nucleolus, in order to better understand their role in adaptive response mechanisms to stress. Thus, we show that nucleolin stress-related gene NUC2 can compensate the environmental stress provided by darkness in nuc1 plants, while nuc2 plants are not able to provide a complete response to red light. These ground control findings, as part of the ESA/NASA Seedling Growth spaceflight experiments will determine the basis for the identification of a genetic background enabling an adaptive advantage for plants in future space experiments.

Keywords: Arabidopsis, Ground controls, Nucleolus, Stress response, RNA-Seq, Spaceflight

Suggested Citation

Manzano, Aránzazu and Villacampa, Alicia and Sáez-Vásquez, Julio and Kiss, John Z. and Medina, F. Javier and Herranz, Raul, The Importance of Earth Reference Controls in Spaceflight – Omics Experiments: Dissecting Transcriptional Responses of Nucleolin Mutants to Red Light Stimulation. Available at SSRN: https://ssrn.com/abstract=3661944 or http://dx.doi.org/10.2139/ssrn.3661944
This version of the paper has not been formally peer reviewed.

Aránzazu Manzano

Centro de Investigaciones Biológicas Margarita Salas (CSIC) ( email )

Spain

Alicia Villacampa

Centro de Investigaciones Biológicas Margarita Salas (CSIC) ( email )

Spain

Julio Sáez-Vásquez

CNRS - , Laboratoire Génome et Développement des Plantes (LGDP) ( email )

John Z. Kiss

University of North Carolina (UNC) at Greensboro - Department of Biology ( email )

P.O.Box 26170
Greensboro, NC 27412
United States

F. Javier Medina

Centro de Investigaciones Biológicas Margarita Salas (CSIC) ( email )

Spain

Raul Herranz (Contact Author)

Centro de Investigaciones Biológicas Margarita Salas (CSIC) ( email )

Spain

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