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2D Ti 3C 2T x Mxene Couples Electrical Stimulation to Promote Proliferation and Neural Differentiation of Neural Stem Cells

28 Pages Posted: 23 Sep 2020 Publication Status: Accepted

See all articles by Rongrong Guo

Rongrong Guo

China Medical University - Department of Clinical Epidemiology

Miao Xiao

Soochow University - Institute for Cardiovascular Science & Department of Cardiovascular Surgery

Wanyu Zhao

3Institute for Regenerative Medicine, Shanghai East Hospital, School of Materials Science and Engineering, Tongji University

Yangnan Hu

Southeast University - Key Laboratory for Developmental Genes and Human Disease

Menghui Liao

Southeast University - Key Laboratory for Developmental Genes and Human Disease

Shengping Wang

Institute for Regenerative Medicine, Shanghai East Hospital, School of Materials Science and Engineering, Tongji University

Xiaowei Yang

Tongji University - Institute for Regenerative Medicine

Renjie Chai

Southeast University - Key Laboratory for Developmental Genes and Human Disease

Mingliang Tang

Soochow University - Institute for Cardiovascular Science

Abstract

Preclinical studies involving stem cells require efficient physiochemical regulations on the fate of such cells. Because of their unique planar structure, excellent conductivity, and flexible surface functionalization, MXenes show potential for modulating stem cell fate. Here, the Ti3C2Tx MXene nanosheets are dispersed on tissue culture polystyrene (TCPS). When primary mouse neural stem cells (NSCs) are cultured on laminin-coated Ti3C2Tx MXene film, they form excellent adhesion, retain their proliferative ability, and show extensive spreading of lamellipodia and filopodia. With respect to their functional activity, NSCs cultured on Ti3C2Tx MXene films form more active and synchronous network than those cultured on TCPS substrates. Moreover, Ti3C2Tx MXene film significantly promotes the neural differentiation and the neurons have longer neurites and greater numbers of branch points and branch tips, suggesting the Ti3C2Tx MXene film also promotes the maturation of NSC-derived neurons. Finally, electrical stimulation coupled with Ti3C2Tx MXene film significantly enhances the proliferation of NSCs. These results indicate that Ti3C2Tx MXene is an efficient interface for the proliferation and neural differentiation of NSC and the maturation of NSC-derived neurons, which expands the potential uses of the MXene family of materials and provides new strategies for stem cell studies.

Keywords: 2D MXene, Stem Cells, Regeneration, neurons, electrical stimulation, differentiation

Suggested Citation

Guo, Rongrong and Xiao, Miao and Zhao, Wanyu and Hu, Yangnan and Liao, Menghui and Wang, Shengping and Yang, Xiaowei and Chai, Renjie and Tang, Mingliang, 2D Ti 3C 2T x Mxene Couples Electrical Stimulation to Promote Proliferation and Neural Differentiation of Neural Stem Cells. Available at SSRN: https://ssrn.com/abstract=3677369 or http://dx.doi.org/10.2139/ssrn.3677369

Rongrong Guo (Contact Author)

China Medical University - Department of Clinical Epidemiology

Shenyang, Liaoning 110004
China

Miao Xiao

Soochow University - Institute for Cardiovascular Science & Department of Cardiovascular Surgery

Wanyu Zhao

3Institute for Regenerative Medicine, Shanghai East Hospital, School of Materials Science and Engineering, Tongji University

Yangnan Hu

Southeast University - Key Laboratory for Developmental Genes and Human Disease

Menghui Liao

Southeast University - Key Laboratory for Developmental Genes and Human Disease

Shengping Wang

Institute for Regenerative Medicine, Shanghai East Hospital, School of Materials Science and Engineering, Tongji University

Xiaowei Yang

Tongji University - Institute for Regenerative Medicine ( email )

Shanghai
China

Renjie Chai

Southeast University - Key Laboratory for Developmental Genes and Human Disease ( email )

Sipailou 2#
Nanjing, Jiangsu Province 210096
China

Mingliang Tang

Soochow University - Institute for Cardiovascular Science ( email )

Suzhou
China

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