Quantitative Analysis of Prednisolone and Aspirin in Synthetic Mixture Using 1st Order Derivative Spectroscopy

IJRAR April 2020, Volume 7, Issue 2

11 Pages Posted: 28 Oct 2020

See all articles by Jay Patel

Jay Patel

Smt.S.M.Shah pharmacy College

Date Written: 2020

Abstract

Current research paper describes highly specific and reproducible 1st order derivative spectroscopic method for quantitative analysis of Prednisolone which is a Glucocorticoid and Aspirin which is non-steroidal anti-inflammatory agent from its synthetic mixture. Present analytical method was developed on Shimadzu double beam spectrophotometer equipped with UV probe 2.42 as software using methanol as solvent. Quantification of Prednisolone was carried out at zero cross over point of Aspirin that is 256 nm and for Aspirin, it was achieved at 243 nm which is zero cross over point of Prednisolone. Method shows linear response in the range of 2-6 µg/mL of Prednisolone and 16-48 µg/mL of Aspirin. Method was found to be accurate with recovery between 98.95 – 99.49 % for PRE and 99.12 – 99.65 % for ASP. The developed method was validated as per ICH Q2 R1 guidelines and was successfully applied for quantitative analysis of synthetic mixture of Prednisolone and Aspirin.

Keywords: Prednisolone, Aspirin, Derivative Spectroscopy, Zero Crossover Point, Analytical Method Validation

JEL Classification: .

Suggested Citation

Patel, Jay, Quantitative Analysis of Prednisolone and Aspirin in Synthetic Mixture Using 1st Order Derivative Spectroscopy (2020). IJRAR April 2020, Volume 7, Issue 2 , Available at SSRN: https://ssrn.com/abstract=3687969

Jay Patel (Contact Author)

Smt.S.M.Shah pharmacy College ( email )

India
09426257764 (Phone)
380026 (Fax)

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