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Involvement of Oral Microbiota in the Gut Microbiota of Colorectal Cancer

23 Pages Posted: 29 Dec 2020

See all articles by Yoshinori Uchino

Yoshinori Uchino

Kagoshima University - Department of Maxillofacial Diagnostic and Surgical Science

Yuichi Goto

Kagoshima University - Department of Maxillofacial Diagnostic and Surgical Science

Yusuke Konishi

Osaka University - Department of Molecular Microbiology

Kan Tanabe

Imamura General Hospital - Digestive Surgery

Hiroko Toda

Fujita Health University Hospital - Breast Surgery

Masumi Wada

Kagoshima University - Department of Digestive Surgery, Breast and Thyroid Surgery

Yoshiaki Kita

Kagoshima University - Department of Digestive Surgery, Breast and Thyroid Surgery

Mahiro Beppu

Kagoshima University - Department of Maxillofacial Diagnostic and Surgical Science

Shinichiro Mori

Kagoshima University - Department of Digestive Surgery, Breast and Thyroid Surgery

Hiroshi Hijioka

Kagoshima University - Department of Maxillofacial Diagnostic and Surgical Science

Takao Otsuka

Kagoshima University - Department of Digestive Surgery, Breast and Thyroid Surgery

Shoji Natsugoe

Kajikionsen Hospital

Eiji Hara

Osaka University - Department of Molecular Microbiology

Tsuyoshi Sugiura

Kagoshima University - Department of Maxillofacial Diagnostic and Surgical Science

More...

Abstract

Background: The oral microbiota is reportedly associated with the gut microbiota and influences colorectal cancer (CRC) progression; however, the details remain unclear.

Methods: This study included 52 patients diagnosed with CRC and 51 healthy controls. Saliva and stool samples were collected, and microbiota were evaluated using 16S rRNA analysis and next-generation sequencing. Comparative analysis was performed on both groups.

Findings: Linear discriminant analysis Effect Size (LEfSe) revealed indigenous oral bacteria, such as Peptostreptococcus, Streptococcus, and Solobacterium spp., were present at a significantly higher relative abundance in CRC patients compared with controls, consistent for both saliva and stool. LEfSe was also performed after the CRC patients were divided into early stage (Stage I, II; n = 26; 50%) and advanced stage (Stage III, IV; n = 26;, 50%) disease. S. moorei was present at a significantly higher relative abundance in the advanced-stage group compared with the early-stage group, again consistent for both saliva and stool.

Interpretation: Among the bacterial species whose relative abundance was significantly higher in CRC patients, P. stomatis, S. anginosus, S. koreensis and S. moorei were apparently supplied from the oral cavity, suggesting indigenous oral bacteria may have promoted initiation of CRC carcinogenesis. Furthermore, S. moorei may influence CRC progression.

Funding Statement: This research was supported by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (19K10361)(15H05045).

Declaration of Interests: All authors declare no interests.

Ethics Approval Statement: The study was conducted in accordance with the “Declaration of Helsinki.” The study protocols were approved by the ethics committee of Kagoshima University. Informed consent was obtained from all the study participants.

Keywords: Microbiota, oral bacteria, colorectal cancer, Peptostreptococcus, Streptococcus, Solobacterium spp.

Suggested Citation

Uchino, Yoshinori and Goto, Yuichi and Konishi, Yusuke and Tanabe, Kan and Toda, Hiroko and Wada, Masumi and Kita, Yoshiaki and Beppu, Mahiro and Mori, Shinichiro and Hijioka, Hiroshi and Otsuka, Takao and Natsugoe, Shoji and Hara, Eiji and Sugiura, Tsuyoshi, Involvement of Oral Microbiota in the Gut Microbiota of Colorectal Cancer. Available at SSRN: https://ssrn.com/abstract=3742276 or http://dx.doi.org/10.2139/ssrn.3742276

Yoshinori Uchino

Kagoshima University - Department of Maxillofacial Diagnostic and Surgical Science ( email )

Sakuragaoka, Kagoshima
Japan

Yuichi Goto

Kagoshima University - Department of Maxillofacial Diagnostic and Surgical Science

Sakuragaoka, Kagoshima
Japan

Yusuke Konishi

Osaka University - Department of Molecular Microbiology ( email )

Japan

Kan Tanabe

Imamura General Hospital - Digestive Surgery ( email )

Kagoshima
Japan

Hiroko Toda

Fujita Health University Hospital - Breast Surgery ( email )

Toyoake, Aichi
Japan

Masumi Wada

Kagoshima University - Department of Digestive Surgery, Breast and Thyroid Surgery ( email )

Kagoshima
Japan

Yoshiaki Kita

Kagoshima University - Department of Digestive Surgery, Breast and Thyroid Surgery ( email )

Kagoshima
Japan

Mahiro Beppu

Kagoshima University - Department of Maxillofacial Diagnostic and Surgical Science ( email )

Sakuragaoka, Kagoshima
Japan

Shinichiro Mori

Kagoshima University - Department of Digestive Surgery, Breast and Thyroid Surgery ( email )

Kagoshima
Japan

Hiroshi Hijioka

Kagoshima University - Department of Maxillofacial Diagnostic and Surgical Science ( email )

Sakuragaoka, Kagoshima
Japan

Takao Otsuka

Kagoshima University - Department of Digestive Surgery, Breast and Thyroid Surgery ( email )

Kagoshima
Japan

Shoji Natsugoe

Kajikionsen Hospital ( email )

Eiji Hara

Osaka University - Department of Molecular Microbiology ( email )

1-1 Yamadaoka
Suita
Osaka, 565-0871
Japan

Tsuyoshi Sugiura (Contact Author)

Kagoshima University - Department of Maxillofacial Diagnostic and Surgical Science ( email )

Sakuragaoka, Kagoshima
Japan

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